Amnion-derived cells express intercellular adhesion molecule-1: regulation by cytokines

Marvin, K.W., Hansen, W.R., Miller, H.C., Eykholt, R.L. and Mitchell, M.D. (1999) Amnion-derived cells express intercellular adhesion molecule-1: regulation by cytokines. Journal Of Molecular Endocrinology, 22 2: 193-205. doi:10.1677/jme.0.0220193


Author Marvin, K.W.
Hansen, W.R.
Miller, H.C.
Eykholt, R.L.
Mitchell, M.D.
Title Amnion-derived cells express intercellular adhesion molecule-1: regulation by cytokines
Journal name Journal Of Molecular Endocrinology   Check publisher's open access policy
ISSN 1479-6813
0952-5041
Publication date 1999-04
Sub-type Article (original research)
DOI 10.1677/jme.0.0220193
Volume 22
Issue 2
Start page 193
End page 205
Total pages 13
Place of publication Bristol, England
Publisher Society for Endocrinology
Language eng
Subject 1103 Clinical Sciences
1115 Pharmacology and Pharmaceutical Sciences
Abstract We have examined the expression of the intercellular adhesion molecule- 1 (ICAM-1) mRNA in primary and established amnion-derived cell cultures and regulation of this expression by tumour necrosis factor-α (TNF-α) and interleukin (IL)-1β. TNF-α (50 ng/ml) and IL-1β (1.0 ng/ml) induced 18- and 11-fold increases respectively in expression of the ICAM-1 mRNA in WISH cells (an amnion epithelium-derived cell line). The increase was detectable within one hour of treatment and peaked by two hours. The protein synthesis inhibitor, cycloheximide (10 μg/ml) did not inhibit this induction. Increased levels of ICAM-1 protein were detected in the cells within 4 h after initiation of treatment with either cytokine. By 16 h of treatment with IL-1β or TNF-α ICAM-1 reached 40 and 73pg/μg cellular protein, representing 6- and 11-fold stimulations respectively. In primary amnion cells, basal expression of ICAM-1 mRNA was undetectable. However, TNF-α (50ng/ml) induced ICAM-1 mRNA within two hours, peak expression being reached between four and eight hours after initiation of treatment. The present report demonstrates for the first time that amnion derived cells can express ICAM-1 and, further, that this expression is regulated by pro-inflammatory cytokines. This has implications for the amnion as a possible source for soluble ICAM-1, for this gene product as a marker for preterm labour, and for participation of the amnion, additional to its reported secretory role, in inflammatory processes of the fetal membranes.
Keyword Tumor-necrosis-factor
Human-endothelial-cells
Intrauterine Growth-retardation
Kappa-B Site
Preterm Labor
Epithelial-cells
Factor-alpha
Transendothelial Migration
Fetal Membranes
Inflammatory cytokines
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Centre for Clinical Research Publications
 
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Created: Thu, 26 Aug 2010, 12:59:38 EST