Regulation of chorion laeve prostaglandin E2 production by epidermal growth factor, protein kinase C activation and calcium

Lundin-Schiller, S. and Mitchell, M.D. (1991) Regulation of chorion laeve prostaglandin E2 production by epidermal growth factor, protein kinase C activation and calcium. Placenta, 12 6: 597-603. doi:10.1016/0143-4004(91)90494-Z


Author Lundin-Schiller, S.
Mitchell, M.D.
Title Regulation of chorion laeve prostaglandin E2 production by epidermal growth factor, protein kinase C activation and calcium
Journal name Placenta   Check publisher's open access policy
ISSN 0143-4004
1532-3102
Publication date 1991-11
Sub-type Article (original research)
DOI 10.1016/0143-4004(91)90494-Z
Volume 12
Issue 6
Start page 597
End page 603
Total pages 7
Place of publication London, England
Publisher W.B. Saunders
Language eng
Subject 1103 Clinical Sciences
1114 Paediatrics and Reproductive Medicine
Formatted abstract
The effects of epidermal growth factor (EGF) phorbol 12-myristate 13-acetate (PMA) A23187 and ionomycin on prostaglandin production by chorion laeve cells in culture for 3 days and 10 days were tested. Experiments were conducted at day 3 because this time the cultures became confluent and again at day 10 because changes have been observed in the biochemical properties of these cells with time in culture. At 3 days of culture the cells did not respond to EGF but at 10 days EGF (10 ng/ml) induced a significant increase in prostaglandin E2 production. PMA (10-9 to 10-6M) induced a significant increase in PGE2 production at both times in culture. The calcium ionophores A23187 and ionomycin were less effective in eliciting a response at either time in culture. Only A23187 (1 μM) induced a significant increase in PGE2 production at day 10 of culture. These data suggest that the presence of functional EGF receptors may increase with time in culture. Furthermore activation of the protein kinase C pathway in the chorion laeve stimulates prostaglandin biosynthesis. On the other hand chorion laeve cell prostaglandin biosynthesis is not responsive to in increases in intracellular calcium induced by mobile ion carriers such as the ionophores used in this study.
Keyword Human Amnion Cells
Human Parturition
Primary Culture
Biosynthesis
Stimulation
Metabolism
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Centre for Clinical Research Publications
 
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Created: Thu, 26 Aug 2010, 12:58:55 EST