Amnion cell prostaglandin E2 stimulatory activity in chorion laeve-conditioned medium

Lundin-Schiller, S., Gibb, W. and Mitchell, M.D. (1990) Amnion cell prostaglandin E2 stimulatory activity in chorion laeve-conditioned medium. Biochimica et Biophysica Acta, 1053 2-3: 151-155. doi:10.1016/0167-4889(90)90007-Z


Author Lundin-Schiller, S.
Gibb, W.
Mitchell, M.D.
Title Amnion cell prostaglandin E2 stimulatory activity in chorion laeve-conditioned medium
Formatted title
Amnion cell prostaglandin E2 stimulatory activity in chorion laeve-conditioned medium
Journal name Biochimica et Biophysica Acta   Check publisher's open access policy
ISSN 0006-3002
Publication date 1990-07
Sub-type Article (original research)
DOI 10.1016/0167-4889(90)90007-Z
Volume 1053
Issue 2-3
Start page 151
End page 155
Total pages 5
Place of publication Amsterdam, The Netherlands
Publisher Elsevier
Language eng
Subject 1103 Clinical Sciences
1114 Paediatrics and Reproductive Medicine
Formatted abstract
In this study we demonstrate the presence of a stimulant(s) to amnion cell prostaglandin (PG) E2 production in chorion-conditioned medium (CCM). The CCM induced a dose-dependent increase in amnion cell PGE2 production. This stimulatory activity was eliminated by heat and protease treatment. Macimal stimulation of amnion PGE2 by CCM did not occur until after 2 h of incubation, and treatment with cycloheximide (1 μg/mg) effectively eliminated the ability of the amnion cells to respond to CCM. Additionally, CCM and arachidonic acid (2-40 μM) were synergistic in their stimulatory actions on amnion PGE2 production. CCM-treated amnion cells recover more quickly from acetylsalicylic acid pretreatment as compared to control. It is concluded that CCM contains a heat-labile protein which stimulates amnion cell PEG2 production by induction of prostaglandin endoperoxide synthase activity.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Centre for Clinical Research Publications
 
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Created: Thu, 26 Aug 2010, 12:58:38 EST