Interleukin-1 stimulates ovarian prostaglandin biosynthesis: Evidence for heterologous contact-independent cell-cell interaction

Kokia, E., Hurwitz, A., Ricciarelli, E., Tedeschi, C., Resnick, C.E., Mitchell, M.D. and Adashi, E.Y. (1992) Interleukin-1 stimulates ovarian prostaglandin biosynthesis: Evidence for heterologous contact-independent cell-cell interaction. Endocrinology, 130 5: 3095-3097. doi:10.1210/en.130.5.3095


Author Kokia, E.
Hurwitz, A.
Ricciarelli, E.
Tedeschi, C.
Resnick, C.E.
Mitchell, M.D.
Adashi, E.Y.
Title Interleukin-1 stimulates ovarian prostaglandin biosynthesis: Evidence for heterologous contact-independent cell-cell interaction
Journal name Endocrinology   Check publisher's open access policy
ISSN 0013-7227
Publication date 1992-05
Sub-type Other
DOI 10.1210/en.130.5.3095
Volume 130
Issue 5
Start page 3095
End page 3097
Total pages 3
Place of publication Bethesda, MA, U.S.A.
Publisher Endocrine Society
Language eng
Subject 1114 Paediatrics and Reproductive Medicine
110306 Endocrinology
1103 Clinical Sciences
Formatted abstract
An increasing body of information now suggests the existence of a complete intraovarian IL-1 system replete with ligands, receptor, and receptor antagonist. Since IL-1 is an established mediator of inflammation and since ovulation may constitute an inflammatory-like reaction, consideration may be given to the possibility that IL-1 may play an intermediary role in the ovulatory process. To further assess the above hypothesis, we have set out to determine whether IL-1 is capable of promoting ovarian prostaglandin biosynthesis, an established component of the ovulatory cascade. Cultured whole ovarian dispersates from immature (25 day old) rats constitutively elaborated major prostaglandin species (PGE2>PGF(2α)) in a cell density- dependent fashion. Treatment with IL-1 produced dose-dependent increments in prostaglandin (PGE2>PGF(2α)) accumulation as compared with untreated controls. Comparable cellular densities of untreated or IL-1β-treated whole ovarian dispersates elaborated substantially more PGE2 as compared with isolated granulosa or theca-interstitial cell preparations suggesting a requirement for cell-cell interaction. Indeed, cell contact-dependent reconstitution experiments involving isolated granulosa and theca- interstitial cells at a projected physiologic ratio of 4:1 revealed synergistic interactions in the elaboration of PGE2 under both basal and IL- 1β-treated circumstances. Identical results were obtained for cell contact- independent heterologous (but not homologous) coculture experiments. Taken together, our present findings reveal optimal basal and IL-1-stimulated ovarian prostaglandin (PGE2>PGF(2α)) biosynthesis to require heterologous, contact-independent, presumably humorally-mediated, cell-cell interaction. These observations along with the demonstration of the gonadotropin-dependent preovulatory induction of ovarian IL-1β gene expression provide strong support for the view that IL-1 may be the centerpiece of an intraovarian regulatory loop concerned with the promotion of the preovulatory cascade.
Keyword Granulosa-Cells
Ovulation
Follicles
Rat
Q-Index Code CX
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Other
Collection: UQ Centre for Clinical Research Publications
 
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Created: Thu, 26 Aug 2010, 12:57:55 EST