Activin A exerts both pro- and anti-inflammatory effects on human term gestational tissues

Keelan, J. A., Zhou, R. L. and Mitchell, M. D. (2000) Activin A exerts both pro- and anti-inflammatory effects on human term gestational tissues. Placenta, 21 1: 38-43. doi:10.1053/plac.1999.0451

Author Keelan, J. A.
Zhou, R. L.
Mitchell, M. D.
Title Activin A exerts both pro- and anti-inflammatory effects on human term gestational tissues
Journal name Placenta   Check publisher's open access policy
ISSN 0143-4004
Publication date 2000-01
Sub-type Article (original research)
DOI 10.1053/plac.1999.0451
Volume 21
Issue 1
Start page 38
End page 43
Total pages 6
Place of publication London, England
Publisher W.B. Saunders
Language eng
Subject 1103 Clinical Sciences
1114 Paediatrics and Reproductive Medicine
Abstract There is a growing appreciation of the importance of activin as a modulator of immune function. The aim of the present study was to determine whether activin A exerts any effects on cytokine and prostaglandin (PG) production by the tissues of pregnancy. Explant cultures were established for amnion, choriodecidual and placental tissues derived from pregnancies delivered at term by Caesarean section (n=5 placentae). Explants were treated with activin A (0.5, 5 and 50 ng/ml) in serum-free Ham's F12/DME media for 24 h (n=3–4 replicates). Production rates of interleukin (IL)-1β, IL-6, IL-8, tumour necrosis factor-α (TNF-α) and PGE2were determined using immunoassay. Differences between treatment groups were analysed by ANOVA followed by Dunnett's test;P< 0.05 was considered to be significant. Amnion IL-6 production exhibited biphasic responses to activin A: at 5 ng/ml activin A, IL-6 production was significantly stimulated (to 246±74.6 per cent of control (mean±sem), while at 50 ng/ml it was significantly inhibited (to 46±7.4 per cent of control). IL-8 and PGE2production by amnion showed significant responses to activin A that were similar to those of IL-6. No significant effects of activin A were observed on choriodecidual and placental IL-6, IL-8 and PGE2production. However, TNF-α production was significantly inhibited by 50 ng/ml activin A in both choriodecidual and placental explants (to 43±9.7 per cent and 51±6.7 per cent of control, respectively). Placental IL-1β production was not altered by treatment with activin A at any concentration. These findings support the concept of activin as an immune modulator in tissues of pregnancy.
Keyword Gonadotropin-releasing-hormone
Human Placental Cells
Maternal Serum
Preterm Labor
Paracrine Regulation
Fetal Membranes
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Centre for Clinical Research Publications
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Created: Thu, 26 Aug 2010, 12:57:22 EST