Regulation of activin A, inhibin A, and follistatin production in human amnion and choriodecidual explants by inflammatory mediators

Keelan, J.A., Zhou, R.L., Evans, L.W., Groome, N.P. and Mitchell, M.D. (2000) Regulation of activin A, inhibin A, and follistatin production in human amnion and choriodecidual explants by inflammatory mediators. Journal of the Society for Gynecologic Investigation, 7 5: 291-296. doi:10.1016/S1071-5576(00)00065-4


Author Keelan, J.A.
Zhou, R.L.
Evans, L.W.
Groome, N.P.
Mitchell, M.D.
Title Regulation of activin A, inhibin A, and follistatin production in human amnion and choriodecidual explants by inflammatory mediators
Journal name Journal of the Society for Gynecologic Investigation   Check publisher's open access policy
ISSN 1071-5576
Publication date 2000-09
Sub-type Article (original research)
DOI 10.1016/S1071-5576(00)00065-4
Volume 7
Issue 5
Start page 291
End page 296
Total pages 6
Place of publication New York, N.Y. U.S.A.
Publisher Elsevier
Language eng
Subject 1103 Clinical Sciences
1114 Paediatrics and Reproductive Medicine
1115 Pharmacology and Pharmaceutical Sciences
Formatted abstract
Objective:
To determine the effects of inflammatory mediators on the production of activin A, inhibin A, and the binding protein follistatin in term amnion and choriodecidual tissues.

Methods:
The effects of interleukin- 1β (IL-1β; 1 ng/mL), tumor necrosis factor-α (TNF-α; 10 ng/mL), and bacterial lipopolysaccharide (LPS; 5 μg/mL) on production rates of activin A, inhibin A, and follistatin by term choriodecidual and amnion membranes in explant culture were determined using specific enzyme-linked immunoabsorbent assays.

Results:
All explants (n = 6 placentas) produced detectable amounts of activin A, inhibin A, and follistatin under basal conditions; choriodecidual production rates were more than tenfold higher than amnion rates. In amnion explants, activin A production was stimulated by IL-1β and TNF-α to 450 ± 155.4% and 531 ± 170.8% of control, respectively (mean ± standard error of the mean; P < .05 by analysis of variance), whereas production of inhibin and follistatin was stimulated to a much more modest extent. Similar responses were observed in the choriodecidual explants. Lipopolysaccharide had no significant effect on amnion activin A production, but stimulated choriodecidual production to 290 ± 34% of control. Lipopolysaccharide exerted only limited effects on inhibin A and follistatin production.

Conclusions:

Treatment with proinflammatory mediators resulted in a preferential increase in activin A production compared with that of inhibin A or follistatin. These findings suggest that inflammation of the gestational membranes could result in increased local activin A production and bioactivity. Copyright (C) 2000 by the Society for Gynecologic Investigation.
Keyword Activin
Inhibin
Follistatin
Cytokines
Parturition
Human Chorionic-gonadotropin
Labor-Associated Chanes
Human Placenta Cells
Human-pregnancy
Maternal Serum
Porcine Follistatin
Dimeric Inhibins
Fetal Membranes
Preterm Labor
Nitric-oxide
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Centre for Clinical Research Publications
 
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Created: Thu, 26 Aug 2010, 12:57:19 EST