Innate inflammatory responses of human decidual cells to periodontopathic bacteria

Keelan, Jeffrey A., Wong, Pui-Mun, Bird, Philip S. and Mitchell, Murray D. (2010). Innate inflammatory responses of human decidual cells to periodontopathic bacteria. In: 39th Annual Scientific Conference of the Society for Reproductive Biology, Melbourne, Vic, Australia, (471.e1-471.e11). 25-28 August, 2008. doi:10.1016/j.ajog.2010.02.031

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Author Keelan, Jeffrey A.
Wong, Pui-Mun
Bird, Philip S.
Mitchell, Murray D.
Title of paper Innate inflammatory responses of human decidual cells to periodontopathic bacteria
Conference name 39th Annual Scientific Conference of the Society for Reproductive Biology
Conference location Melbourne, Vic, Australia
Conference dates 25-28 August, 2008
Journal name American Journal of Obstetrics and Gynecology   Check publisher's open access policy
Place of Publication St. Louis, MO, U.S.A.
Publisher Mosby
Publication Year 2010
Sub-type Fully published paper
DOI 10.1016/j.ajog.2010.02.031
ISSN 0002-9378
1097-6868
Volume 202
Issue 5
Start page 471.e1
End page 471.e11
Total pages 11
Collection year 2011
Language eng
Formatted Abstract/Summary
Objective:
The purpose of this study was to test the hypothesis that periodontopathic bacteria exert potent proinflammatory effects in human decidua.

Study Design:

The immunostimulatory effects of Gram-positive and negative periodontopathic bacteria and their lipopolysaccharides were tested in human decidual cell cultures in comparison with Escherichia coli. Cytokine production was measured by enzyme-linked immunosorbent assay; inflammatory gene expression was measured by oligonucleotide arrays and quantitative real time-polymerase chain reaction.

Results:
All bacteria that were tested elicited an inflammatory response, although concentration-dependence and efficacy varied considerably with organism and culture. Lipopolysaccharides were more potent stimuli than intact bacterial cells, although bacteria exerted greater effects at high concentrations. Of 112 genes on the arrays, 18 genes were stimulated significantly by one or more lipopolysaccharide preparation.

Conclusion:
The ability of periodontopathic bacteria to stimulate a decidual inflammatory response is highly variable and partly dependent on the presence and structure of constituent lipopolysaccharides. This adds to our understanding of the causal association between periodontal disease and preterm birth. Crown Copyright © 2010.
Subjects 0604 Genetics
1114 Paediatrics and Reproductive Medicine
Keyword Decidua
Inflammation
Periodontopathic bacteria
Preterm Birth
Toll-like Receptors
Necrosis-factor-alpha
Porphyromonas-gingivalis
Preterm Birth
Actinobacillus-actinomycetemcomitans
Fusobacterium-nucleatum
Pregnancy Outcomes
Tnf-alpha
Disease
Lipopolysaccharides
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published under "Basic Science: Obstetrics". "Presented at the 39th Annual Scientific Conference of the Society for Reproductive Biology, Melbourne, VIC, Australia, Aug. 25-28, 2008."

Document type: Conference Paper
Collections: UQ Centre for Clinical Research Publications
Official 2011 Collection
 
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Created: Thu, 26 Aug 2010, 12:57:04 EST