Regulation of interleukin (IL)-6 and IL-8 production in an amnion-derived cell line by cytokines, growth factors, glucocorticoids, and phorbol esters

Keelan, J. A., Sato, T. and Mitchell, M. D. (1997). Regulation of interleukin (IL)-6 and IL-8 production in an amnion-derived cell line by cytokines, growth factors, glucocorticoids, and phorbol esters. In: American Journal of Reproductive Immunology. 44th Annual Meeting of the Society-for-Gynecologic-Investigation, San Diego, California, U.S.A., (272-278). 19-22 March, 1997.

Author Keelan, J. A.
Sato, T.
Mitchell, M. D.
Title of paper Regulation of interleukin (IL)-6 and IL-8 production in an amnion-derived cell line by cytokines, growth factors, glucocorticoids, and phorbol esters
Conference name 44th Annual Meeting of the Society-for-Gynecologic-Investigation
Conference location San Diego, California, U.S.A.
Conference dates 19-22 March, 1997
Proceedings title American Journal of Reproductive Immunology   Check publisher's open access policy
Journal name American Journal of Reproductive Immunology   Check publisher's open access policy
Place of Publication Copenhagen, Denmark
Publisher Munksgaard
Publication Year 1997
Sub-type Fully published paper
ISSN 1046-7408
0271-7352
1600-0897
Volume 38
Issue 4
Start page 272
End page 278
Total pages 6
Language eng
Formatted Abstract/Summary
PROBLEM:
To determine whether amnion cells produce interleukin (IL)-6 and -8 and thus may contribute to the high concentrations of these cytokines in amniotic fluid at term.

METHOD OF STUDY:

Amnion-derived WISH cells were treated in culture with stimuli over 16 hr, and IL-6 and IL-8 concentrations in the conditioned media were measured by enzyme-linked immunosorbent assay or bioassay (IL-6 only).

RESULTS:

IL-8 production was ~ 5-fold higher than that of IL-6 under basal and stimulated conditions. Significant (by Dunnett's test after analysis of variance) stimulation of production of both cytokines was achieved by IL-1β (> 0.2 ng/ml), TNFα (> 10 ng/ml), and the phorbol ester, phorbol 12-myristate 13-acetate (> 2 nM), over a 16-hr culture period. Epidermal growth factor at 10 ng/ml induced a small increase in production of IL-8, but not of IL-6, whereas bacterial lipopolysaccharide had minimal effects on production of either cytokine. Basal and cytokine-stimulated IL-6 and IL-8 production was inhibited by dexamethasone at concentrations equal to or greater than 1 nM.

CONCLUSION:

These findings suggest that amnion may be a significant contributor to the IL-6 and IL-8 content of amniotic fluid, and that WISH cells may be a suitable model for the study of cytokine production by amnion epithelial cells.
Subjects 1103 Clinical Sciences
1114 Paediatrics and Reproductive Medicine
1115 Pharmacology and Pharmaceutical Sciences
Keyword Amnion
Cytokines
Infection
Parturition
Pregnancy
Expression
Tumor-necrosis-factor
Prostaglandin Production
Preterm Labor
Inflammatory cytokines
Fluid Interluekin-6
Human-pregnancy
Wish Cells
Q-Index Code E1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Conference Paper
Collection: UQ Centre for Clinical Research Publications
 
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Created: Thu, 26 Aug 2010, 12:56:46 EST