Interleukin (IL)-6 and IL-8 production by human amnion: Regulation by cytokines, growth factors, glucocorticoids, phorbol esters, and bacterial lipopolysaccharide

Keelan, J.A., Sato, T. and Mitchell, M.D. (1997) Interleukin (IL)-6 and IL-8 production by human amnion: Regulation by cytokines, growth factors, glucocorticoids, phorbol esters, and bacterial lipopolysaccharide. Biology of Reproduction, 57 6: 1438-1444. doi:10.1095/biolreprod57.6.1438


Author Keelan, J.A.
Sato, T.
Mitchell, M.D.
Title Interleukin (IL)-6 and IL-8 production by human amnion: Regulation by cytokines, growth factors, glucocorticoids, phorbol esters, and bacterial lipopolysaccharide
Journal name Biology of Reproduction   Check publisher's open access policy
ISSN 0006-3363
Publication date 1997-12-01
Sub-type Article (original research)
DOI 10.1095/biolreprod57.6.1438
Volume 57
Issue 6
Start page 1438
End page 1444
Total pages 7
Place of publication Madison, WI, U.S.A.
Publisher Society for the Study of Reproduction
Language eng
Subject 1103 Clinical Sciences
1114 Paediatrics and Reproductive Medicine
1115 Pharmacology and Pharmaceutical Sciences
Abstract Amniotic fluid at term contains high concentrations of interleukin (IL)- 6 and IL-8. The source of these cytokines has not been identified, although the fetal membranes (amnion and chorion) are likely contributors. Amnion cytokine production was investigated by using amnion cells isolated by enzymatic digestion (from placentas delivered at term before labor) and cultured in vitro. IL-6 and IL-8 were measured in conditioned media by ELISA. Amnion cells produced detectable amounts of both IL-6 and IL-8 throughout the 7-day culture period. The ratio of IL-8 to IL-6 was approximately 5:1, similar to the ratio found in amniotic fluid. Production of both IL-6 and IL- 8 was stimulated in a concentration-dependent fashion by interleukin-1β (0.1-10 ng/ml), tumor necrosis factor-α (1-100 ng/ml), and bacterial lipopolysaccharide (0.1-10 μg/ml), and also by 100 nM phorbol 12-myristate 13-acetate. Epidermal growth factor (1-25 ng/ml) had only minimal effects on amnion cytokine production. Dexamethasone (10 nM) inhibited IL-6/-8 production by approximately 50% throughout the culture period. Production of IL-6/-8 by cultured amniotic fibroblasts, which under basal conditions was much lower than that by epithelial cells, was regulated by all the agents tested in a fashion similar to that of the epithelial cells. These findings suggest that the amnion contributes to the pool of IL-6 and IL-8 in amniotic fluid. We speculate that amnion-derived cytokines might have functions during normal human parturition that are distinct from their conventional roles as inflammatory mediators.
Keyword Tumor-necrosis-factor
Human Fetal Membranes
Inflammatory cytokines
Preterm Labor
Prostaglandin Production
Fluid Interleukin-6
Gestational Tissues
Cell Biosynthesis
Human-pregnancy
Expression
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Centre for Clinical Research Publications
 
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