Misidentification of prostamides as prostaglandins

Glass, M., Hong, J.W., Sato, T.A. and Mitchell, M.D. (2005) Misidentification of prostamides as prostaglandins. Journal of Lipid Research, 46 7: 1364-1368. doi:10.1194/jlr.C500006-JLR200


Author Glass, M.
Hong, J.W.
Sato, T.A.
Mitchell, M.D.
Title Misidentification of prostamides as prostaglandins
Journal name Journal of Lipid Research   Check publisher's open access policy
ISSN 0022-2275
Publication date 2005-07
Sub-type Article (original research)
DOI 10.1194/jlr.C500006-JLR200
Open Access Status DOI
Volume 46
Issue 7
Start page 1364
End page 1368
Total pages 5
Place of publication New York, N.Y. U.S.A.
Publisher Lipid Research
Language eng
Subject 1103 Clinical Sciences
1114 Paediatrics and Reproductive Medicine
1115 Pharmacology and Pharmaceutical Sciences
Abstract Prostaglandins and endogenous cannabinoid metabolites share the same lipid backbone with differing polar head groups at exactly the position through which a large molecule is attached to provide antigenicity and thus raise antisera. Hence, we hypothesized that antisera raised against prostaglandins linked to a large molecule such as BSA at the carboxyl functional group would also recognize endogenous cannabinoid metabolites and lead to highly misleading interpretations of data. We found major cross-reactivity of commercial antisera raised to prostaglandins with endocannabinoid metabolites. Furthermore, in a well-characterized cell line (WISH) or primary amnion tissue explants, endocannabinoid treatment led to increased production of endocannabinoid metabolites as opposed to primary prostaglandins. This was apparent only after separation of products by thin-layer chromatography, because they measured as prostaglandins by radioimmunoassay. These findings have major implications for our interpretation of data in situations in which these prostaglandin-like molecules are formed, and they stress the need for chromatographic or spectrometric confirmation of prostaglandin production detected by antibody-based methods. Copyright © 2005 by the American Society for Biochemistry and Molecular Biology, Inc.
Keyword Ethanolamides
Prostamides
Endocananabinoids
Anandamide
Cannabinoid Receptors
Cyclooxygenase-2 Expression
E-2 Ethanolamide
Cb1 Receptor
Binds
Brain
Cells
Metabolism
Activation
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Centre for Clinical Research Publications
 
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Created: Thu, 26 Aug 2010, 12:53:06 EST