Cyclooxygenase products formed by primary cultures of cells from human chorion laeve: Influence of steroids

Gibb, W., Riopel, L., Collu, R., Ducharme, J.R., Mitchell, M.D. and Lavoie, J.C. (1988) Cyclooxygenase products formed by primary cultures of cells from human chorion laeve: Influence of steroids. Canadian Journal of Physiology And Pharmacology, 66 6: 788-793. doi:10.1139/y88-125


Author Gibb, W.
Riopel, L.
Collu, R.
Ducharme, J.R.
Mitchell, M.D.
Lavoie, J.C.
Title Cyclooxygenase products formed by primary cultures of cells from human chorion laeve: Influence of steroids
Journal name Canadian Journal of Physiology And Pharmacology   Check publisher's open access policy
ISSN 0008-4212
Publication date 1988-06-01
Sub-type Article (original research)
DOI 10.1139/y88-125
Volume 66
Issue 6
Start page 788
End page 793
Total pages 5
Place of publication Ottawa, ON, Canada
Publisher National Research Council Canada
Language eng
Subject 1103 Clinical Sciences
0601 Biochemistry and Cell Biology
Formatted abstract
Cells were isolated from human chorion laeve obtained at term (38-40 weeks gestation) by elective caesarean section and were maintained in primary culture for 1 week in defined media supplemented with 10% fetal calf serum. The production of various cyclooxygenase products by the cultures was examined. Little or no prostaglandin (PG) F(2α), 6-keto-PGF(1α), thromboxane B2, or 13,14-dihydro-15-keto-PGF(2α) was found. In contrast, the cells produced PGE2 which was low on day 0, increased during culture to a maximum on day 1 or 2, then declined to low levels. When cells were grown in the presence of media containing cortisol, dexamethasone, progesterone, and estradiol (at 10-7 or 10-9 M), the glucocorticoids (at 10-7 and 10-9 M), but not estrogen or progesterone, markedly inhibited the increase in PGE2 output. There was no difference in the protein content and thymidine incorporation of cells grown in the presence of glucocorticoids when compared with controls. This inhibitory effect was not sensitive to cycloheximide (1 μg/mL) indicating protein synthesis may not be involved in the process. These studies indicate that PGE2 is the major prostaglandin formed by primary cultures of chorion laeve and that prostaglandin metabolism in the chorion is sensitive to glucocorticoid inhibition.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Centre for Clinical Research Publications
 
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Created: Thu, 26 Aug 2010, 22:53:00 EST