Cytokines regulate prostaglandin H synthase-1 transcription in human amnion-derived cells

Eykholt, R. L., Hansen, W. R., Potter, S., Marvin, K. W. and Mitchell, M. D. (1999) Cytokines regulate prostaglandin H synthase-1 transcription in human amnion-derived cells. Prostaglandins Leukotrienes and Essential Fatty Acids, 61 5: 323-329. doi:10.1054/plef.1999.0108


Author Eykholt, R. L.
Hansen, W. R.
Potter, S.
Marvin, K. W.
Mitchell, M. D.
Title Cytokines regulate prostaglandin H synthase-1 transcription in human amnion-derived cells
Journal name Prostaglandins Leukotrienes and Essential Fatty Acids   Check publisher's open access policy
ISSN 0952-3278
1532-2823
Publication date 1999-11
Sub-type Article (original research)
DOI 10.1054/plef.1999.0108
Volume 61
Issue 5
Start page 323
End page 329
Total pages 7
Place of publication Edinburgh ; New York
Publisher Churchill Livingstone
Language eng
Subject 0604 Genetics
1114 Paediatrics and Reproductive Medicine
Abstract We have isolated the prostaglandin H synthase-1 (PGHS-1) promoter from the human amnion cell line WISH by long template PCR. The fragment was 1124 base pairs in length and shared a 96% sequence identity with the sequence in GenBank. The putative transcription start site is located 18 bp upstream of the start codon. The sequence is TATA-less, but contains multiple Sp-1 sites and a GC box at -132. The fragment was subcloned into the promoterless reporter construct pBLCAT3 to produce the promoter reporter construct pPGHS1CAT. pPGHS1CAT expression in amnion-derived AV3 cells was inhibited by the pro-inflammatory cytokines tumor necrosis factor-alpha (TNF-α) and interleukin 1-beta (IL-1β). PGHS-1 mRNA levels however, were unchanged over a 16-h time course with either treatment. These results suggest that PGHS-1 transcription is regulated in a negative manner by cytokines in human amnion-derived cells.
Keyword Necrosis-factor-alpha
Osteoblastic Mc3t3-e1 Cells
Messenger-rna Levels
Endoperoxide Synthase
Arachidonic-acid
G/H Synthase
Inducible Cyclooxygenase
Gene Disruption
Phorbol Ester
Wish Cells
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Centre for Clinical Research Publications
 
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Created: Thu, 26 Aug 2010, 12:52:31 EST