Proteomics: Recent applications and new technologies

Elrick, Mollisa M., Walgren, Jennie L., Mitchell, Michael D. and Thompson, David C. (2006) Proteomics: Recent applications and new technologies. Basic & Clinical Pharmacology & Toxicology, 98 5: 432-441. doi:10.1111/j.1742-7843.2006.pto_391.x


Author Elrick, Mollisa M.
Walgren, Jennie L.
Mitchell, Michael D.
Thompson, David C.
Title Proteomics: Recent applications and new technologies
Journal name Basic & Clinical Pharmacology & Toxicology   Check publisher's open access policy
ISSN 1742-7843
1742-7835
Publication date 2006-05
Sub-type Critical review of research, literature review, critical commentary
DOI 10.1111/j.1742-7843.2006.pto_391.x
Volume 98
Issue 5
Start page 432
End page 441
Total pages 10
Place of publication Oxford, England
Publisher Blackwell Munksgaard for the Nordic Pharmacological Society
Language eng
Subject 1103 Clinical Sciences
1004 Medical Biotechnology
Abstract Interest in proteomics as a tool for drug development and a myriad of other applications continues to expand at a rapid rate. Proteomic analyses have recently been conducted on tissues, biofluids, subcellular components and enzymatic pathways as well as various disease and toxicological states, in both animal models and man. In addition, several recent studies have attempted to integrate proteomics data with genomics and/or metabonomics data in a systems biology approach. The translation of proteomic technology and bioinformatics tools to clinical samples, such as in the areas of disease and toxicity biomarkers, represents one of the major opportunities and challenges facing this field. An ongoing challenge in proteomics continues to be the analysis of the serum proteome due to the vast number and complexity of proteins estimated to be present in this biofluid. Aside from the removal of the most abundant proteins, a number of interesting approaches have recently been suggested that may help reduce the overall complexity of serum analysis. In keeping with the increasing interest in applications of proteomics, the tools available for proteomic analyses continue to improve and expand. For example, enhanced tools (such as software and labeling procedures) continue to be developed for the analysis of 2D gels and protein quantification. In addition, activity-based probes are now being used to tag, enrich and isolate distinct sets of proteins based on enzymatic activity. One of the most active areas of development involves microarrays. Antibody-based microarrays have recently been released as commercial products while numerous additional capture agents (e.g. aptamers) and many additional types of microarrays are being explored. © Basic & Clinical Pharmacology & Toxicology 2006. All rights reserved.
Keyword 2-Dimensional Gel-electrophoresis
Tandem Mass-spectrometry
Liquid-chromatography
Quantitative Proteomics
Potential Biomarkers
Protein microarrays
Software Packages
Cancer Markers
Identification
Serum
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Critical review of research, literature review, critical commentary
Collection: UQ Centre for Clinical Research Publications
 
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Created: Thu, 26 Aug 2010, 12:52:28 EST