The regulation of arachidonate lipoxygenase metabolite formation in cells derived from intrauterine tissues

Edwin, S.S., Thai, D., LaMarche, S., Branch, D.W. and Mitchell, M.D. (1995) The regulation of arachidonate lipoxygenase metabolite formation in cells derived from intrauterine tissues. Prostaglandins Leukotrienes and Essential Fatty Acids, 52 4: 229-233. doi:10.1016/0952-3278(95)90041-1


Author Edwin, S.S.
Thai, D.
LaMarche, S.
Branch, D.W.
Mitchell, M.D.
Title The regulation of arachidonate lipoxygenase metabolite formation in cells derived from intrauterine tissues
Journal name Prostaglandins Leukotrienes and Essential Fatty Acids   Check publisher's open access policy
ISSN 0952-3278
1532-2823
Publication date 1995-04-01
Sub-type Article (original research)
DOI 10.1016/0952-3278(95)90041-1
Volume 52
Issue 4
Start page 229
End page 233
Total pages 5
Place of publication Edinburgh ; New York
Publisher Churchill Livingstone
Language eng
Subject 0604 Genetics
1114 Paediatrics and Reproductive Medicine
Formatted abstract
Products of arachidonic acid (AA) metabolism via the lipoxygenase pathways may have key roles in the maintenance of pregnancy and the onset of labor. We have determined whether calcium ionophores can modulate the rate of biosynthesis within the uterus of five important arachidonate lipoxygenase metabolites, i.e. leukotriene B4 (LTB4), LTC4, 5-hydroxyeicosatetraenoic acid (5-HETE), 12-HETE, and 15-HETE. Amnion, chorion, and decidual cells were isolated, grown to confluence and incubated with ionomycin. The production of LTB4, LTC4, 5-HETE, 12-HETE, and 15-HETE was determined using specific radioimmunoassays. Cell-specific, concentration-related stimulatory actions of ionomycin on 5-HETE, 12-HETE, 15-HETE, and LTC4 but not LTB4 production were found. A23187 had effects similar to ionomycin. Hence elevation of intracellular calcium levels can result in enhanced intrauterine production of arachidonate lipoxygenase metabolites that may affect pregnancy outcome.
Keyword Platelet-activating Factor
Human-Fetal Membranes
Human Chorion Laeve
Amniotic-fluid
Preterm Labor
5-Hydroxyeicosatetraenoic Acid
Prostaglandin Production
Inflammatory Mediators
Human Parturition
Uterine Decidua
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Centre for Clinical Research Publications
 
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Created: Thu, 26 Aug 2010, 22:52:03 EST