Inflammatory mediators regulate interleukin-8 production by cultured gestational tissues: Evidence for a cytokine network at the chorio-decidual interface

Dudley, D.J., Trautman, M.S. and Mitchell, M.D. (1993) Inflammatory mediators regulate interleukin-8 production by cultured gestational tissues: Evidence for a cytokine network at the chorio-decidual interface. Journal of Clinical Endocrinology & Metabolism, 76 2: 404-410. doi:10.1210/jc.76.2.404


Author Dudley, D.J.
Trautman, M.S.
Mitchell, M.D.
Title Inflammatory mediators regulate interleukin-8 production by cultured gestational tissues: Evidence for a cytokine network at the chorio-decidual interface
Journal name Journal of Clinical Endocrinology & Metabolism   Check publisher's open access policy
ISSN 0021-972X
Publication date 1993-02
Sub-type Article (original research)
DOI 10.1210/jc.76.2.404
Volume 76
Issue 2
Start page 404
End page 410
Total pages 7
Place of publication Bethesda, MD, U.S.A.
Publisher Endocrine Society
Language eng
Subject 1103 Clinical Sciences
1114 Paediatrics and Reproductive Medicine
Abstract The physiology of parturition, and the pathophysiology of preterm labor, is incompletely understood. Infection of gestational tissues may account for a significant proportion of women who experience preterm labor. Interleukin- 8 (IL-8), or neutrophil-activating protein (NAP-1), is a potent chemotactic and activating factor for neutrophils and has been implicated in the pathogenesis of inflammatory injury to skin and lung, but has yet to be described in gestational tissues. Cultured chorion and decidual cells obtained from normal human pregnancies were used to evaluate whether these tissues produce IL-8 basally and in response to inflammatory cytokines. As measured by specific IL-8 RIA, chorion and decidual cells produce IL-8 constitutively and in response to IL-1β and tumor necrosis factor. Cotreatment of chorion cells or decidual cells with IL-1β and actinomycin D or cycloheximide abrogated IL-8 production. Northern blot analysis confirmed that IL-1β stimulation of chorion and decidual cells resulted in increased IL-8 messenger RNA expression. Our data support the concept that a complex cytokine network between maternal and fetal gestational tissues exists, and that activation of inflammatory cytokine production in these tissues, including IL-8, likely contributes to the pathophysiology of infection- induced preterm labor.
Keyword Onset
Neutrophil Chemotactic Factor
Tumor Necrosis Factor
Preterm Labor
Prostaglandin Biosynthesis
Human Amnion
Intraamniotic Infection
Cells
Parturition
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Centre for Clinical Research Publications
 
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Created: Thu, 26 Aug 2010, 12:51:36 EST