Biosynthesis of interleukin-6 by cultured human chorion laeve cells: Regulation by cytokines

Dudley, D.J., Trautman, M.S., Edwin, S.S., Lundin-Schiller, S. and Mitchell, M.D. (1992) Biosynthesis of interleukin-6 by cultured human chorion laeve cells: Regulation by cytokines. Journal of Clinical Endocrinology & Metabolism, 75 4: 1081-1086. doi:10.1210/jc.75.4.1081

Author Dudley, D.J.
Trautman, M.S.
Edwin, S.S.
Lundin-Schiller, S.
Mitchell, M.D.
Title Biosynthesis of interleukin-6 by cultured human chorion laeve cells: Regulation by cytokines
Journal name Journal of Clinical Endocrinology & Metabolism   Check publisher's open access policy
ISSN 0021-972X
Publication date 1992-10
Sub-type Article (original research)
DOI 10.1210/jc.75.4.1081
Volume 75
Issue 4
Start page 1081
End page 1086
Total pages 6
Place of publication Bethesda, MD, U.S.A.
Publisher Endocrine Society
Language eng
Subject 1103 Clinical Sciences
1114 Paediatrics and Reproductive Medicine
Abstract Intrauterine infection is an important cause of preterm labor and delivery and is characterized by increased production of inflammatory cytokines by gestational tissues. We evaluated the biosynthesis of the inflammatory cytokine interleukin-6 (IL-6) by human chorion laeve cells and its regulation by other cytokines essential to the inflammatory process. We found that cultured chorion cells secrete IL-6 in the presence of growth medium supplemented only with 10% fetal calf serum. IL-1β, tumor necrosis factor, and lipopolysaccharide all induced a significant concentration-dependent stimulation of IL-6 production by chorion cells. The concentration range of each cytokine tested (0.1-10 ng/mL) is within the range of values found in the amniotic fluid of women destined to deliver preterm due to infection of gestational tissues. Additionally, treatment of chorion cells with IL-1β in combination with actinomycin-D or cycloheximide attenuated the stimulatory action of IL-1β on IL-6 production. Northern blot analysis of total RNA from cultured chorion cells stimulated with IL-1β demonstrated that IL-6 mRNA increases over time. Our data suggest that IL-6 is produced by human fetal chorion in response to infection of maternal gestational tissues. In conjunction with other inflammatory mediators, fetally derived IL-6 may play a role in the pathophysiology of preterm labor due to infection.
Keyword Hepatocyte-Stimulating Factor
Tumor Necrosis Factor
Preterm Labor
Prostaglandin Biosynthesis
Inflammatory Mediators
Decidual cells
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Centre for Clinical Research Publications
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Created: Thu, 26 Aug 2010, 12:51:33 EST