Pathophysiology of antiphospholipid antibodies: absence of prostaglandin-mediated effects on cultured endothelium

Dudley, D. J., Mitchell, M. D. and Branch, D. W. (1990). Pathophysiology of antiphospholipid antibodies: absence of prostaglandin-mediated effects on cultured endothelium. In: American Journal of Obstetrics and Gynecology:. 8th Annual Meeting of The American Gynecological and Obstetrical Society, Hot Springs, Va. U.S.A, (953-959). 7-9 September, 1989.

Author Dudley, D. J.
Mitchell, M. D.
Branch, D. W.
Title of paper Pathophysiology of antiphospholipid antibodies: absence of prostaglandin-mediated effects on cultured endothelium
Conference name 8th Annual Meeting of The American Gynecological and Obstetrical Society
Conference location Hot Springs, Va. U.S.A
Conference dates 7-9 September, 1989
Proceedings title American Journal of Obstetrics and Gynecology:   Check publisher's open access policy
Journal name American Journal of Obstetrics and Gynecology   Check publisher's open access policy
Place of Publication St. Louis, Mo. U.S.A.
Publisher Mosby
Publication Year 1990
Sub-type Fully published paper
ISSN 0002-9378
Volume 162
Issue 4
Start page 953
End page 959
Total pages 6
Language eng
Abstract/Summary Antiphospholipid antibodies are associated with recurrent pregnancy loss and thrombosis. Studies suggest that antiphospholipid antibodies may inhibit the production of prostacyclin by vascular tissues. We incubated sera from women with moderate to high levels of antiphospholipid antibodies with primary human umbilical vein endothelial cell cultures. Intact confluent, subconfluent, hydrogen peroxide-damaged, and mechanically damaged endothelial cell monolayers were evaluated for the production of the prostacyclin metabolite 6-keto-prostaglandin F(1α) in the presence of sera that were positive and negative for antiphospholipid antibodies. All sera were assayed for baseline concentrations of 6-keto-prostaglandin F(1α) before incubation with endothelial monolayers. Additionally, the extent of binding of antiphospholipid antibodies to intact and damaged endothelium was studied by immunofluorescent techniques and enzyme-linked immunosorbent assay. Our results indicate that prostacyclin production is not impaired by sera containing antiphospholipid antibodies, regardless of the condition of the endothelium. Further, we were unable to demonstrate binding of immunoglobulin from sera containing antiphospholipid antibodies to intact or damaged endothelium. However, the baseline concentration of 6-keto-prostaglandin F(1α) was nearly fourfold higher in sera containing antiphospholipid antibodies. The finding of elevated baseline serum concentrations of prostacyclin metabolite in patients with antiphospholipid antibodies suggests a condition of long-term stimulation or altered metabolism of prostacyclin.
Subjects 0604 Genetics
1114 Paediatrics and Reproductive Medicine
Q-Index Code E1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Conference Paper
Collection: UQ Centre for Clinical Research Publications
 
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