Amniotic fluid interleukin-10 (IL-10) concentrations during pregnancy and with labor

Dudley, D.J., Hunter, C., Mitchell, M.D. and Varner, M.W. (1997) Amniotic fluid interleukin-10 (IL-10) concentrations during pregnancy and with labor. Journal of Reproductive Immunology, 33 2: 147-156. doi:10.1016/S0165-0378(97)00020-X

Author Dudley, D.J.
Hunter, C.
Mitchell, M.D.
Varner, M.W.
Title Amniotic fluid interleukin-10 (IL-10) concentrations during pregnancy and with labor
Journal name Journal of Reproductive Immunology   Check publisher's open access policy
ISSN 0165-0378
Publication date 1997-06
Sub-type Article (original research)
DOI 10.1016/S0165-0378(97)00020-X
Volume 33
Issue 2
Start page 147
End page 156
Total pages 10
Place of publication Amsterdam, The Netherlands
Publisher Elsevier Science
Language eng
Subject 1103 Clinical Sciences
1114 Paediatrics and Reproductive Medicine
1115 Pharmacology and Pharmaceutical Sciences
Abstract To determine if amniotic fluid interleukin-10 (IL-10) concentrations are elevated in women with labor, either at term or preterm, and in the setting of infection-associated preterm labor, amniotic fluid samples were collected from women: (1) at term, not in labor (n = 42); at term, in labor (n = 56), preterm contractions, undelivered within 1 week (n = 22), and preterm labor, delivered within 1 week (n = 31). IL-10 concentrations were assayed in each sample via ELISA (Pharmingen, San Diego, CA). In a subsequent analysis, 8 women with preterm labor associated with chorioamnionitis were matched for gestational age with women experiencing preterm contractions (undelivered within 7 days) and preterm labor (delivered within 7 days) and amniotic fluid IL-10 concentrations compared. Approximately 40-70% of amniotic fluid samples obtained from women in each group had detectable IL-10. However, there were no significant differences in amniotic-fluid IL-10 concentrations among the patients. While 1 of 8 patients with chorioamnionitis had amniotic fluid IL-10 concentrations greater than 300 pg/ml, there were no statistically significant differences among the matched samples. Amniotic fluid IL-10 concentrations were not elevated in women with term labor, preterm labor, or chorioamnionitis. This finding contrasts with the elevated concentrations of pro-inflammatory cytokines and chemokines such as interleukin-1, tumor necrosis factor-α, IL-6, IL-8, MIP-1α, and GROα reported in previous studies. Because we did not detect elevations of the key anti-inflammatory cytokine IL-10 in amniotic fluid of women with infection-associated preterm labor, we suggest that anti-inflammatory processes in this setting may be attenuated.
Keyword Interleukin-10
Preterm Labor
Macrophage Inflammatory Protein-1-alpha
Receptor Antagonist
Intraamniotic Infection
Intrauterine Infection
Premature labor
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Centre for Clinical Research Publications
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Created: Thu, 26 Aug 2010, 12:51:10 EST