A human endometrial explant system: validation and potential applications

Dudley, D.J., Hatasaka, H.H., Branch, D.W., Hammond, E. and Mitchell, M.D. (1992) A human endometrial explant system: validation and potential applications. American Journal of Obstetrics and Gynecology, 167 6: 1774-1780.

Author Dudley, D.J.
Hatasaka, H.H.
Branch, D.W.
Hammond, E.
Mitchell, M.D.
Title A human endometrial explant system: validation and potential applications
Journal name American Journal of Obstetrics and Gynecology   Check publisher's open access policy
ISSN 0002-9378
Publication date 1992-12
Sub-type Article (original research)
Volume 167
Issue 6
Start page 1774
End page 1780
Total pages 7
Place of publication St. Louis, Mo. U.S.A.
Publisher Mosby
Language eng
Subject 0604 Genetics
1114 Paediatrics and Reproductive Medicine
Formatted abstract
Our objective was to describe an in vitro explant system to study the regulation of prostaglandin production by human endometrium.


Segments of late-luteal-phase endometrium were obtained aseptically at the time of endometrial sampling. The endometrium was cut into 1 mm3 pieces and applied to the polycarbonate membrane of tissue-culture-well inserts for 12-well plates (Costar Transwell cell culture chamber inserts, 0.4 μm pore size). After placing the well inserts, culture medium was carefully applied. The explants were incubated at 37°C in 5% carbon dioxide in air, and the culture medium was changed daily.

Electron microscopic examination and lactate dehydrogenase determinations of the explants revealed cellular viability for ≤ 5 days of culture. Endometrial explants responded to treatment with interleukin-1β and tumor necrosis factor by a concentration-dependent increase in the production of prostaglandin E2. Costimulation of late luteal endometrial explants with interleukin-1β (10 ng/ml) and progesterone (10-6 mol/L) resulted in variable production of prostaglandin E2, suggesting that the histologic examination of the endometrium does not necessarily reflect the functional properties of the endometrium.

Our data show that when used with human endometrial tissue this explant system maintains tissue viability and responsiveness for ≤ 5 days. This approach to explant methods is simple and provides a flexible model to study the regulation of the production of bioactive substances by human endometrial tissue.
Keyword Endometrium
Explant Culture
Prostaglandin E2
Prostaglandin Production
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Centre for Clinical Research Publications
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Citation counts: TR Web of Science Citation Count  Cited 16 times in Thomson Reuters Web of Science Article | Citations
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Created: Thu, 26 Aug 2010, 12:51:02 EST