Inflammatory cytokine mRNA in human gestational tissues: Implications for term and preterm labor

Dudley, Donald J., Collmer, Deborah, Mitchell, Murray D. and Trautman, Michael S. (1996) Inflammatory cytokine mRNA in human gestational tissues: Implications for term and preterm labor. Journal of the Society for Gynecologic Investigation, 3 6: 328-335. doi:10.1016/S1071-5576(96)00042-1


Author Dudley, Donald J.
Collmer, Deborah
Mitchell, Murray D.
Trautman, Michael S.
Title Inflammatory cytokine mRNA in human gestational tissues: Implications for term and preterm labor
Journal name Journal of the Society for Gynecologic Investigation   Check publisher's open access policy
ISSN 1071-5576
Publication date 1996-11
Sub-type Article (original research)
DOI 10.1016/S1071-5576(96)00042-1
Volume 3
Issue 6
Start page 328
End page 335
Total pages 8
Place of publication New York, N.Y. U.S.A.
Publisher Elsevier
Language eng
Subject 1103 Clinical Sciences
1114 Paediatrics and Reproductive Medicine
Formatted abstract
OBJECTIVE:
To determine if inflammatory cytokine mRNA in gestational tissues is present only in the setting of infection-associated preterm labor or under several other clinical conditions.

METHODS:
Human gestational tissues were collected from 51 women experiencing 1) term cesarean delivery without labor, 2) normal term vaginal delivery, 3) preterm cesarean delivery without labor, 4) preterm vaginal delivery without chorioamnionitis, and 5) preterm vaginal delivery with concomitant chorioamnionitis. Decidua, chorion, and amnion were isolated, total RNA from each tissue was extracted, and the presence of inflammatory cytokine mRNA was determined by polymerase chain reaction. Interleukin (IL)-1β, IL-6, IL-8, and tumor necrosis factor-α mRNA was detected using specific oligonucleotide primers.

RESULTS:
Interleukin- 1β mRNA was rarely found in tissues preterm without labor but was readily detected in both maternal and fetal tissues after labor, regardless of gestational age. Interleukin-6 mRNA was rare in tissues from the nonlaboring patient but was found in almost all tissues after labor. Interleukin-8 mRNA was detected in all tissues at term, both in nonlaboring and laboring patients. Tumor necrosis factor-α mRNA was detected in only 20-50% of tissues after labor, and was rarely detected in the absence of labor.

CONCLUSIONS:

Inflammatory cytokine mRNA is commonly expressed in human gestational tissues after normal labor and preterm labor with or without associated intrauterine infection. There was no difference in the pattern of expression of mRNA inflammatory cytokine in women who did not have clinically evident intrauterine infection.
Keyword Inflammatory cytokines
Preterm Labor
Decidua
Chorion
Intrauterine infection
Tumor necrosis factor
Amniotic-Fluid Interleukin-6
Chorion Laeve Cells
Prostaglandin Biosynthesis
Intra-Amniotic Infection
Intrauterine Infection
Molecular-cloning
Decidual cells
Messenger-rna
Mediators
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Centre for Clinical Research Publications
 
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Created: Thu, 26 Aug 2010, 12:50:50 EST