Is interleukin-3 important in antiphospholipid anti body-mediated pregnancy failure?

Chamley, L.W., Konarkowska, B., Duncalf, A.M., Mitchell, M.D. and Johnson, P.M. (2001) Is interleukin-3 important in antiphospholipid anti body-mediated pregnancy failure?. Fertility and Sterility, 76 4: 700-706. doi:10.1016/S0015-0282(01)01984-7


Author Chamley, L.W.
Konarkowska, B.
Duncalf, A.M.
Mitchell, M.D.
Johnson, P.M.
Title Is interleukin-3 important in antiphospholipid anti body-mediated pregnancy failure?
Journal name Fertility and Sterility   Check publisher's open access policy
ISSN 0015-0282
1556-5653
Publication date 2001-10
Sub-type Article (original research)
DOI 10.1016/S0015-0282(01)01984-7
Volume 76
Issue 4
Start page 700
End page 706
Total pages 7
Place of publication New York, N.Y. U.S.A.
Publisher Elsevier
Language eng
Subject 1103 Clinical Sciences
1114 Paediatrics and Reproductive Medicine
1115 Pharmacology and Pharmaceutical Sciences
Formatted abstract
Objective:
To investigate the effect of interleukin-3 (IL-3) on trophoblast proliferation and expression of β2-glycoprotein I.

Design:
In vitro cell culture using primary trophoblasts and the cell lines Jeg-3, Jar, and BeWo.

Setting:
Department of Obstetrics and Gynaecology, University of Auckland. Patient(s): Women with normal pregnancies. Intervention(s): Increasing amounts of IL-3 were added to cultures of primary human trophoblasts, cell lines, or cells treated with a proliferation inhibiting antiphospholipid-like antibody. RNA was extracted from primary human trophoblasts or cell lines.

Main Outcome Measure(s):
We examined basal and IL-3-stimulated cellular proliferation by [3H] thymidine incorporation assay and secretion of β2-glycoprotein I into culture medium by semiquantative immunoblot analysis. Reverse transcriptase-polymerase chain reaction analysis was used to demonstrate the presence of IL-3 receptor transcripts.

Result(s):
The IL-3 treatment did not induce proliferation of highly purified primary trophoblast cultures or cell lines but did induce proliferation of contaminating CD45+ cells in trophoblast cultures. The IL-3 did not overcome the antiproliferative effect of an antiphospholipid-like monoclonal antibody on trophoblast. Secretion of β2-glycoprotein I by trophoblast cultures was time dependent but unaltered by IL-3 treatment.

Conclusion(s):
Our results question the proposed importance of IL-3 in antiphospholipid antibody-mediated fetal death. © 2001 by American Society for Reproductive Medicine.
Keyword Antiphospholipid antibodies
Beta(2)-glycoprotein I
Interleukin-3
Stillbirth
Miscarriage
Trophoblast
Human Trophoblast
Fetal loss
GM-CSF
Antibodies
Anticardiolpin
Expression
Receptors
Beta-2-Glycoprotein-I
Differentiation
Proliferation
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Centre for Clinical Research Publications
 
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Created: Thu, 26 Aug 2010, 12:49:24 EST