Is interleukin-3 important in antiphospholipid anti body-mediated pregnancy failure?

Chamley, L.W., Konarkowska, B., Duncalf, A.M., Mitchell, M.D. and Johnson, P.M. (2001) Is interleukin-3 important in antiphospholipid anti body-mediated pregnancy failure?. Fertility and Sterility, 76 4: 700-706. doi:10.1016/S0015-0282(01)01984-7

Author Chamley, L.W.
Konarkowska, B.
Duncalf, A.M.
Mitchell, M.D.
Johnson, P.M.
Title Is interleukin-3 important in antiphospholipid anti body-mediated pregnancy failure?
Journal name Fertility and Sterility   Check publisher's open access policy
ISSN 0015-0282
Publication date 2001-10
Sub-type Article (original research)
DOI 10.1016/S0015-0282(01)01984-7
Volume 76
Issue 4
Start page 700
End page 706
Total pages 7
Place of publication New York, N.Y. U.S.A.
Publisher Elsevier
Language eng
Subject 1103 Clinical Sciences
1114 Paediatrics and Reproductive Medicine
1115 Pharmacology and Pharmaceutical Sciences
Formatted abstract
To investigate the effect of interleukin-3 (IL-3) on trophoblast proliferation and expression of β2-glycoprotein I.

In vitro cell culture using primary trophoblasts and the cell lines Jeg-3, Jar, and BeWo.

Department of Obstetrics and Gynaecology, University of Auckland. Patient(s): Women with normal pregnancies. Intervention(s): Increasing amounts of IL-3 were added to cultures of primary human trophoblasts, cell lines, or cells treated with a proliferation inhibiting antiphospholipid-like antibody. RNA was extracted from primary human trophoblasts or cell lines.

Main Outcome Measure(s):
We examined basal and IL-3-stimulated cellular proliferation by [3H] thymidine incorporation assay and secretion of β2-glycoprotein I into culture medium by semiquantative immunoblot analysis. Reverse transcriptase-polymerase chain reaction analysis was used to demonstrate the presence of IL-3 receptor transcripts.

The IL-3 treatment did not induce proliferation of highly purified primary trophoblast cultures or cell lines but did induce proliferation of contaminating CD45+ cells in trophoblast cultures. The IL-3 did not overcome the antiproliferative effect of an antiphospholipid-like monoclonal antibody on trophoblast. Secretion of β2-glycoprotein I by trophoblast cultures was time dependent but unaltered by IL-3 treatment.

Our results question the proposed importance of IL-3 in antiphospholipid antibody-mediated fetal death. © 2001 by American Society for Reproductive Medicine.
Keyword Antiphospholipid antibodies
Beta(2)-glycoprotein I
Human Trophoblast
Fetal loss
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Centre for Clinical Research Publications
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Citation counts: TR Web of Science Citation Count  Cited 23 times in Thomson Reuters Web of Science Article | Citations
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Created: Thu, 26 Aug 2010, 12:49:24 EST