Assay of plasma endogenous inhibitor(s) of prostaglandin synthase using an enzyme incubation - radioimmunoassay method

Brennecke, S.P., Humphreys, J., Turnbull, A.C. and Mitchell, M.D. (1982) Assay of plasma endogenous inhibitor(s) of prostaglandin synthase using an enzyme incubation - radioimmunoassay method. Prostaglandins Leukotrienes and Medicine, 8 6: 615-634.

Author Brennecke, S.P.
Humphreys, J.
Turnbull, A.C.
Mitchell, M.D.
Title Assay of plasma endogenous inhibitor(s) of prostaglandin synthase using an enzyme incubation - radioimmunoassay method
Journal name Prostaglandins Leukotrienes and Medicine   Check publisher's open access policy
ISSN 0262-1746
Publication date 1982-06
Sub-type Article (original research)
Volume 8
Issue 6
Start page 615
End page 634
Total pages 20
Place of publication Edinburgh ; New York
Publisher Churchill Livingstone
Language eng
Subject 0604 Genetics
1114 Paediatrics and Reproductive Medicine
Formatted abstract
A simple, reliable method with validations is described from the routine measurement of endogenous inhibitor(s) of prostaglandin synthase in blood plasma. Appropriate dilutions of plasma samples are incubated with bovine seminal vesicle prostaglandin synthase, sodium arachidonate and reduced glutathione. Prostaglandin E2 production is then quantitated by radioimmunoassay. The relative inhibitory potency of plasma samples is determined by comparison of prostaglandin E2 content in sample incubations with that of control incubations containing no plasma dilution. Possible applications and the suitability of this methodological approach are discussed.


Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Centre for Clinical Research Publications
 
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Created: Thu, 26 Aug 2010, 12:48:32 EST