Differential effects of serum constituents on apoptosis induced by the cyclopentenone prostaglandin 15-deoxy-Delta(12,14)-prostaglandin J(2) in WISH epithelial cells

Berry, E.B.E., Sato, T.A., Mitchell, M.D., Gilmour, R.S. and Helliwell, R.J.A. (2004) Differential effects of serum constituents on apoptosis induced by the cyclopentenone prostaglandin 15-deoxy-Delta(12,14)-prostaglandin J(2) in WISH epithelial cells. Prostaglandins Leukotrienes and Essential Fatty Acids, 71 3: 191-197. doi:10.1016/j.plefa.2004.04.001


Author Berry, E.B.E.
Sato, T.A.
Mitchell, M.D.
Gilmour, R.S.
Helliwell, R.J.A.
Title Differential effects of serum constituents on apoptosis induced by the cyclopentenone prostaglandin 15-deoxy-Delta(12,14)-prostaglandin J(2) in WISH epithelial cells
Journal name Prostaglandins Leukotrienes and Essential Fatty Acids   Check publisher's open access policy
ISSN 0952-3278
1532-2823
Publication date 2004-09
Sub-type Article (original research)
DOI 10.1016/j.plefa.2004.04.001
Volume 71
Issue 3
Start page 191
End page 197
Total pages 7
Place of publication Edinburgh ; New York
Publisher Churchill Livingstone
Language eng
Subject 0604 Genetics
1114 Paediatrics and Reproductive Medicine
Formatted abstract
Cyclopentenone prostaglandins, Δ12-PGJ2 and 15d-PGJ2, have potent anti-tumour and anti-inflammatory activities, and have been shown to induce apoptosis in amnion-derived WISH cells. In this study, we have investigated the protective effects of serum and its constituents (growth factors and albumin) on Δ12-PGJ2 and 15d-PGJ2-induced apoptosis in WISH cells. Serum (0.5% w/v) was protective against both Δ12-PGJ2 and 15d-PGJ 2-induced apoptosis. This was not due to the presence of serum-derived growth factors (EGF, IGF-1 and IGF-2), since they had no significant effect on 15d-PGJ2-induced cell death. In contrast, IGF-1 partially inhibited etoposide-induced apoptosis, confirming the presence of a functional IGF-1 receptor signalling system. Albumin was identified as the key survival factor in serum, since albumin and delipidated albumin exhibited the same level of protection from 15d-PGJ2-induced apoptosis as serum itself. The potential for serum albumin to regulate the bioactivity of cyclopentenone PGs may be of considerable importance in pathological conditions where roles for cyclopentenone PGs have been identified. © 2004 Elsevier Ltd. All rights reserved.
Keyword Activated Receptor-gamma
IGF-I Receptor
Prostanoid Receptors
Signaling Pathway
Survival Factor
Human-plasma
Fatty-acids
Ppar-gamma
Albumin
Growth
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Centre for Clinical Research Publications
 
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