Genetic transformation of sweet sorghum

Birch, Robert G. and Raghuwanshi, Anshu (2010) Genetic transformation of sweet sorghum. Plant Cell Reports, 29 9: 997-1005. doi:10.1007/s00299-010-0885-x


Author Birch, Robert G.
Raghuwanshi, Anshu
Title Genetic transformation of sweet sorghum
Journal name Plant Cell Reports   Check publisher's open access policy
ISSN 0721-7714
1432-203X
Publication date 2010-09
Sub-type Article (original research)
DOI 10.1007/s00299-010-0885-x
Volume 29
Issue 9
Start page 997
End page 1005
Total pages 9
Place of publication Berlin, Germany ; New York, U.S.A.
Publisher Springer International
Collection year 2011
Language eng
Subject C1
820404 Sorghum
8204 Summer Grains and Oilseeds
1001 Agricultural Biotechnology
Formatted abstract
Sweet sorghum has substantial potential as a biofuel feedstock, with advantages in some environments over alternatives such as sugarcane or maize. Gene technologies are likely to be important to achieve yields sufficient for food, fuel and fibre production from available global croplands, but sorghum has proven difficult to transform. Tissue culture recalcitrance and poor reproducibility of transformation protocols remain major challenges for grain sorghum, and there has been no reported success for sweet sorghum. Here we describe a repeatable transformation system for sweet sorghum, based on (1) optimized tissue culture conditions for embryogenic callus production with >90% regenerability in 12-week-old calli, and (2) an effective selection regimen for hygromycin resistance conferred by a Ubi-hpt transgene following particle bombardment. Using this method, we have produced sixteen independent transgenic lines from multiple batches at an overall efficiency of 0.09% transformants per excised immature embryo. Co-expression frequency of a non-selected luciferase reporter was 62.5%. Transgene integration and expression were confirmed in T0 and T1 plants by Southern analysis and luciferase assays. This success using the major international sweet sorghum cultivar Ramada provides a foundation for molecular improvement of sweet sorghum through the use of transgenes. Factors likely to be important for success with other sweet sorghum cultivars are identified.
© 2010 Springer-Verlag.

Keyword Sorghum Bicolor
Hygromycin resistance
Luciferase
Immature embryos
Regeneration
Microprojectile Bombardment
Agrobacterium-mediated Transformation
Tissue
Bicolor
Immature Embryos
Somatic Embryogenesis.
Microprojectile Bombardment
Callus Induction
Transgenic Sorghum
Plant-regeneration
High-tannin sorghums
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
School of Biological Sciences Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 10 times in Thomson Reuters Web of Science Article | Citations
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Created: Tue, 24 Aug 2010, 12:29:45 EST by Joni Taylor on behalf of School of Biological Sciences