Extraction and dissolution of starch from rice and sorghum grains for accurate structural analysis

Syahariza, Z. A., Li, Enpeng and Hasjim, Jovin (2010) Extraction and dissolution of starch from rice and sorghum grains for accurate structural analysis. Carbohydrate Polymers, 82 1: 14-20. doi:10.1016/j.carbpol.2010.04.014

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Author Syahariza, Z. A.
Li, Enpeng
Hasjim, Jovin
Title Extraction and dissolution of starch from rice and sorghum grains for accurate structural analysis
Journal name Carbohydrate Polymers   Check publisher's open access policy
ISSN 0144-8617
1879-1344
Publication date 2010-08-02
Sub-type Article (original research)
DOI 10.1016/j.carbpol.2010.04.014
Volume 82
Issue 1
Start page 14
End page 20
Total pages 7
Place of publication London
Publisher Applied Science/Elsevier
Collection year 2011
Language eng
Formatted abstract
A complete dissolution without degradation and that removes non-starch components is important for accurate characterisation of starch molecular structure. Current milling and dissolution methods have limitations including incomplete dissolution and molecular degradation. An improved multi-step extraction/dissolution method was devised and tested, involving cryo-grinding, protease pre-treatment, dissolution in dimethyl sulfoxide solution containing 0.5% (w/w) LiBr (DMSO/LiBr) at 80 degrees C, centrifugation, ethanol precipitation, and, finally, re-dissolution in DMSO/LiBr at 80 degrees C. Cryo-grinding and dissolution in DMSO/LiBr were found to have negligible effects on the size distribution of rice starch molecules measured by size-exclusion chromatography (SEC). The peaks of non-starch components were removed or separated from the amylose and amylopectin peaks in the size distributions of rice and sorghum starches. The amylopectin component had a larger hydrodynamic radius than that obtained by conventional wet-milling and also that obtained without protease pre-treatment, suggesting that molecular degradation and aggregation were reduced with the new method. This new extraction/dissolution method allows a more accurate structural analysis of starch molecules from grains than conventional treatments. (C) 2010 Elsevier Ltd. All rights reserved.
Keyword Starch
Dissolution
Starch structure
Size exclusion chromatography
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
Centre for Nutrition and Food Sciences Publications
 
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Created: Sun, 22 Aug 2010, 00:10:38 EST