Comparative efficiency of subcellular targeting signals for expression of a toxic protein in sugarcane

Jackson, Mark A., Nutt, Kerry A., Hassall, Rachael and Rae, Anne L. (2010) Comparative efficiency of subcellular targeting signals for expression of a toxic protein in sugarcane. Functional Plant Biology, 37 8: 785-793. doi:10.1071/FP09243


Author Jackson, Mark A.
Nutt, Kerry A.
Hassall, Rachael
Rae, Anne L.
Title Comparative efficiency of subcellular targeting signals for expression of a toxic protein in sugarcane
Journal name Functional Plant Biology   Check publisher's open access policy
ISSN 1445-4408
1445-4416
Publication date 2010-07-26
Sub-type Article (original research)
DOI 10.1071/FP09243
Volume 37
Issue 8
Start page 785
End page 793
Total pages 9
Editor Rana Munns
Place of publication Melbourne, Australia
Publisher CSIRO
Collection year 2011
Language eng
Subject C1
820304 Sugar
0607 Plant Biology
Formatted abstract
Transgenic sugarcane plants (Saccharum hybrid) have been proposed as a production platform for recombinant proteins, including those providing pathogen resistance as well as high value therapeutic proteins. For the in planta production of proteins that are potentially toxic, a careful consideration of subcellular location is required in order to optimise yield and to avoid detrimental interaction with plant cellular processes. In this study, avidin, a glycoprotein that is potentially toxic to cells because of its high affinity to the co-vitamin biotin, was used to test the effectiveness of a range of targeting signals. Accumulation of avidin was directed to the apoplast, endoplasmic reticulum and to the lytic and delta type vacuoles. Although targeting to the delta vacuole resulted in the highest yields of avidin, these plants developed a biotin deficient phenotype, indicating that this targeting was not fully effective in protecting cellular biotin pools. Similar problems were also observed when avidin was retained in the endoplasmic reticulum. When avidin was targeted to the lytic vacuole using the targeting signal from the sugarcane legumain, plants remained phenotypically normal; however, avidin was predominantly detected as a degraded product due to site-specific limited proteolysis in the vacuole. For avidin and other potentially toxic products, this lytic vacuole targeting signal may be useful if stability within this proteolytic environment can be improved.
© 2010 CSIRO.

Keyword Biofactory
Endoplasmic reticulum
Saccharum
Vacuole
Biotin-binding proteins
Insect pests
Gm-csf
Transgenic sugarcane
Confers resistance
Avidin
Plants
Gene
Leaves
Transformation
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
School of Biological Sciences Publications
 
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Created: Sun, 15 Aug 2010, 00:05:16 EST