New plasmids and putative virulence factors from the draft genome of an Australian clinical isolate of photorhabdus asymbiotica

Wilkinson, Paul, Paszkiewicz, Konrad, Moorhouse, Alex, Szubert, Jan M., Beatson, Scott, Gerrard, John, Waterfield, Nicholas R. and Ffrench-Constant, Nicholas R. (2010) New plasmids and putative virulence factors from the draft genome of an Australian clinical isolate of photorhabdus asymbiotica. FEMS Microbiology Letters, 309 2: 136-143. doi:10.1111/j.1574-6968.2010.02030.x


Author Wilkinson, Paul
Paszkiewicz, Konrad
Moorhouse, Alex
Szubert, Jan M.
Beatson, Scott
Gerrard, John
Waterfield, Nicholas R.
Ffrench-Constant, Nicholas R.
Title New plasmids and putative virulence factors from the draft genome of an Australian clinical isolate of photorhabdus asymbiotica
Journal name FEMS Microbiology Letters   Check publisher's open access policy
ISSN 0378-1097
1574-6968
Publication date 2010-08
Sub-type Article (original research)
DOI 10.1111/j.1574-6968.2010.02030.x
Volume 309
Issue 2
Start page 136
End page 143
Total pages 8
Place of publication Oxford, England
Publisher Blackwell Publishing
Collection year 2011
Language eng
Subject 0605 Microbiology
1108 Medical Microbiology
Abstract Clinical isolates of Photorhabdus asymbiotica have been recovered from patients in both the United States of America and Australia, and the full sequence of P. asymbiotica ATCC43949 from the United States has been reported recently. In contrast to other bacteria in the genus that only infect insects, P. asymbiotica strains are able to infect both insects and humans. Using a combination of Solexa (Illumina) and 454 Life Sciences (Roche) sequence data in different assembly pipelines, we report on a draft genome sequence of a strain of P. asymbiotica recovered from a patient from Kingscliff, Australia. The best assembly yielded an N50 scaffold size of 288 627 base pairs (bp) with 488.6% of the predicted genome covered by scaffolds over 100 000 bp. One of the central differences found between this Australian isolate and the US isolate is the presence of an additional plasmid, pPAA3. This plasmid is similar to pCRY from Yersinia pestis, the causative agent of bubonic plague, and the presence of pPAA3 may account for the increased virulence of Australian isolates both against tissue culture cells and infected patients. The genome of the Kingscliff strain also contains several genomic differences from the US isolate, whose potential significance in virulence against both humans and insects is discussed. © 2010 Federation of European Microbiological Societies
Keyword Genome sequencing
De Novo Sequence assembly
Photorhabdus asymbiotica
Bioinformatics
Illumina
454 Pyrosequencing
IV Secretion Systems
Protein Families
Pathogen
Database
Toxin
Luminescens
Xenorhabdus
Infection
Bacteria
Sequence
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Article first published online: 8 JUN 2010

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
School of Chemistry and Molecular Biosciences
 
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Created: Sun, 01 Aug 2010, 00:07:09 EST