Requirement for phosphoinositide 3-kinase in insulin-stimulated GLUT4 translocation in 3T3-L1 adipocytes

Kotani, K., Carozzi, A. J., Sakaue, H., Hara, K., Robinson, L. J., Clark, S. F., Yonezawa, K., James, D. E. and Kasuga, M. (1995) Requirement for phosphoinositide 3-kinase in insulin-stimulated GLUT4 translocation in 3T3-L1 adipocytes. Biochemical and Biophysical Research Communications, 209 1: 343-348. doi:10.1006/bbrc.1995.1509


Author Kotani, K.
Carozzi, A. J.
Sakaue, H.
Hara, K.
Robinson, L. J.
Clark, S. F.
Yonezawa, K.
James, D. E.
Kasuga, M.
Title Requirement for phosphoinositide 3-kinase in insulin-stimulated GLUT4 translocation in 3T3-L1 adipocytes
Journal name Biochemical and Biophysical Research Communications   Check publisher's open access policy
ISSN 0006-291X
Publication date 1995-04-06
Sub-type Article (original research)
DOI 10.1006/bbrc.1995.1509
Volume 209
Issue 1
Start page 343
End page 348
Total pages 6
Place of publication New York
Publisher Academic Press
Language eng
Subject 0601 Biochemistry and Cell Biology
060108 Protein Trafficking
060111 Signal Transduction
Abstract Insulin stimulates glucose transport in muscle and fat cells by inducing the redistribution of a specific glucose transporter, GLUT4 from intracellular vesicles to the cell surface. Phosphoinositide (PI) 3-kinase has been implicated as a key intermediate in insulin-stimulated glucose transport by studies that have examined the effects of wortmannin and LY294002, which are thought to be specific inhibitors of this enzyme. However, the specificity of these compounds for PI 3-kinase has recently been questioned. Epidermal growth factor, which activates mitogen-activated protein kinase in mouse 3T3-L1 adipocytes, has now been shown to have no effect on PI 3-kinase activity or GLUT4 translocation in these cells. Furthermore, microinjection of a dominant negative mutant of the 85-kDa subunit of PI 3-kinase, which lacks a binding site for the catalytic 110-kDa subunit, inhibited GLUT4 translocation induced by insulin in 3T3-L1 adipocytes; microinjection of the wild-type protein had no effect. These observations indicate that PI 3-kinase is necessary for insulin-induced GLUT4 translocation and glucose transport in adipocytes.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Australian Institute for Bioengineering and Nanotechnology Publications
 
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Created: Mon, 19 Jul 2010, 10:47:37 EST by Thelma Whitbourne on behalf of Aust Institute for Bioengineering & Nanotechnology