A conserved distal segment of the mouse CSF-1 receptor promoter is required for maximal expression of a reporter gene in macrophages and osteoclasts of transgenic mice

Ovchinnikov, Dmitry A., DeBats, Claire E. E., Sester, David P., Sweet, Matthew J. and Hume, David A. (2010) A conserved distal segment of the mouse CSF-1 receptor promoter is required for maximal expression of a reporter gene in macrophages and osteoclasts of transgenic mice. Journal of Leukocyte Biology, 87 5: 815-822.


Author Ovchinnikov, Dmitry A.
DeBats, Claire E. E.
Sester, David P.
Sweet, Matthew J.
Hume, David A.
Title A conserved distal segment of the mouse CSF-1 receptor promoter is required for maximal expression of a reporter gene in macrophages and osteoclasts of transgenic mice
Journal name Journal of Leukocyte Biology   Check publisher's open access policy
ISSN 0741-5400
1938-3673
Publication date 2010-05
Sub-type Article (original research)
DOI 10.1189/jlb.0809557
Volume 87
Issue 5
Start page 815
End page 822
Total pages 8
Place of publication Bethesda, MD., U.S.
Publisher Federation of American Societies for Experimental Biology
Collection year 2011
Language eng
Formatted abstract Csf1r mRNA in adult mice is expressed in cells of the macrophage lineage, and during development, it is also expressed from a separate promoter in placental trophoblast cells. This mouse trophoblast promoter sequence is conserved across species, but human trophoblasts actually initiate transcription from a separate promoter 20 kb upstream, which is not conserved in rodents. A 7.2-kb fragment of the mouse Csf1r genomic DNA, including the 3.5-kb promoter, the first coding exon and downstream intron, is sufficient to direct reproducible position- and copy number-independent expression of an EGFP reporter in vitro and in vivo. In this study, we have examined the consequence of removal of the 150-bp fragment encompassing the conserved trophoblast promoter region in the context of the 7.2-kb promoter on reporter gene expression in transgenic mice. The deletion ablated expression in the placenta but also abolished expression in multinucleated OCL and reduced expression in macrophages. RT-PCR analyses of Csf1r mRNA revealed that mouse OCL use another promoter within this region, distinct from that used in placental trophoblasts, to generate an alternative 5′UTR.
© Society for Leukocyte Biology.
Keyword Transgenic
Knockout Mice
Mononuclear Phagocyte System
Transcription Factor
Csf1r Gene
In-vitro
Inflammation
Cells
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published online before print February 1, 2010,

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
Institute for Molecular Bioscience - Publications
 
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Created: Sun, 18 Jul 2010, 00:04:16 EST