Chimeric hepatitis B core antigen particles containing B- and Th-epitopes of human papillomavirus type 16 E7 protein induce specific antibody and T-helper responses in immunised mice

Tindle, Robert W., Herd, Karen, Londoño, Patricia, Fernando, Germain J. P., Chatfield, Steven N., Malcolm, Karen and Dougan, Gordon (1994) Chimeric hepatitis B core antigen particles containing B- and Th-epitopes of human papillomavirus type 16 E7 protein induce specific antibody and T-helper responses in immunised mice. Virology, 200 2: 547-557. doi:10.1006/viro.1994.1217


Author Tindle, Robert W.
Herd, Karen
Londoño, Patricia
Fernando, Germain J. P.
Chatfield, Steven N.
Malcolm, Karen
Dougan, Gordon
Title Chimeric hepatitis B core antigen particles containing B- and Th-epitopes of human papillomavirus type 16 E7 protein induce specific antibody and T-helper responses in immunised mice
Journal name Virology
ISSN 0042-6822; 096-0341
Publication date 1994
Sub-type Article (original research)
DOI 10.1006/viro.1994.1217
Volume 200
Issue 2
Start page 547
End page 557
Total pages 11
Place of publication California, USA
Publisher Academic Press
Collection year 1994
Language eng
Subject 1108 Medical Microbiology
Formatted abstract
The use of hepatitis B core antigen (HBcAg) as an immunogenic delivery vehicle for foreign epitopes has been reported. Here we report the insertion of DNA sequences encoding immunodominant linear B-epitopes and a "universal" T-helper epitope of human papillomavirus (HPV) type 16 E7 transforming protein into the full-length HBcAg gene cloned into an inducible bacterial expression plasmid (pPN1.0). The resulting chimeric proteins assembled into particles which were highly immunogenic. Mice immunised with particles containing one or two of the three E7 B-epitopes under consideration produced strong epitope-specific antibody responses with both IgG and IgG2a components which recognised eukaryotic E7. T-proliferative responses were elicited to the E7 T-epitope as well as HBeAg T-epitope(s). Lymph node cells from immunised mice produced IL-2 and IL-4 when specifically recalled in vitro, indicating stimulation of both Th1 and Th2 helper cell compartments. Since HBcAg particles can be administered in adjuvant acceptable for human application and can elicit mucosal responses after nasal or oral immunisation, these results have implications for vaccine design in HPV16-associated anogenital cancer.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
 
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