Insecticides cytotoxicity and CYP1A1 induction in primary human and rat hepatocytes cultures

G. de Sousa, F. Fontaine, M. Pralavorio, D. Botta-Fridlund, Y. Letreut and R. Rahmani (1997). Insecticides cytotoxicity and CYP1A1 induction in primary human and rat hepatocytes cultures. In: F. M. Marano, D. J. Benford, J. V. Castell and Ch. A. Reinhardt, Toxicology in vitro. International Workshop on In Vitro Toxicology, Papendal, The Netherlands, (451-453). 24 - 28 June, 1996. doi:10.1016/S0887-2333(97)00061-1

Author G. de Sousa
F. Fontaine
M. Pralavorio
D. Botta-Fridlund
Y. Letreut
R. Rahmani
Title of paper Insecticides cytotoxicity and CYP1A1 induction in primary human and rat hepatocytes cultures
Conference name International Workshop on In Vitro Toxicology
Conference location Papendal, The Netherlands
Conference dates 24 - 28 June, 1996
Proceedings title Toxicology in vitro   Check publisher's open access policy
Journal name Toxicology in Vitro   Check publisher's open access policy
Place of Publication Oxford
Publisher Elsevier Science
Publication Year 1997
Sub-type Fully published paper
DOI 10.1016/S0887-2333(97)00061-1
ISSN 0887-2333
Editor F. M. Marano
D. J. Benford
J. V. Castell
Ch. A. Reinhardt
Volume 11
Issue 5
Start page 451
End page 453
Total pages 7
Language eng
Abstract/Summary With the increasing demand for insecticide products, the question of their safety has become one of the serious world public health concerns. The capability of compounds belonging to the major insecticide families [such as chlorinated hydrocarbons (DDT), carbamates (carbaryl: CBR), organophosphorus compounds (malathion, tetrachlorvinfos: MAL, TCV), pyrethroids (cypermethrin: CPR) and benzoylurea (diflubenzuron: DFU)] in inducing CYP1 Al in rat and human hepatocyte cultures has been tested. Cells were treated during 3 days with six non-toxic increasing doses of insecticides and CYP1A1 expression was assessed by ethoxyresorufin O-deethylase (EROD) activity and by Northern blots. A strong and dose-dependent induction was observed with TCV and DFU, both in human (approx. five- and sevenfold over control, respectively) and in rat hepatocytes (approx. sevenfold). However, EROD induction and CYP/A1 mRNA levels were correlated for DFU but not for TCV, suggesting different regulation mechanisms for PCYP1A1 gene expression by the two compounds. CBR and CPR exerted less induction in both cell types (approx. 2.5-fold induction compared with approximately 16-fold for 3-methylcholanthrene), whereas DDT and MAL showed no action on human hepatocytes but decreased EROD activity in rat cells. Finally, cytotoxicity studies performed using the MTT and the neutral red tests demonstrated significant differences between insecticides.
Subjects 111506 Toxicology (incl.Clinical Toxicology)
Q-Index Code E1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Conference Paper
Collection: Institute for Molecular Bioscience - Publications
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 18 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 21 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Wed, 14 Jul 2010, 09:34:21 EST by Laura McTaggart on behalf of Institute for Molecular Bioscience