Involvement of Ets, rel and Sp1-like proteins in lipopolysaccharide-mediated activation of the HIV-1 LTR in macrophage

Sweet, M. J., Stacey, K. J., Ross, I. L., Ostrowski, M. C. and Hume, D. A. (1998) Involvement of Ets, rel and Sp1-like proteins in lipopolysaccharide-mediated activation of the HIV-1 LTR in macrophage. Journal of Inflammation, 48 2: 67-83.

Author Sweet, M. J.
Stacey, K. J.
Ross, I. L.
Ostrowski, M. C.
Hume, D. A.
Title Involvement of Ets, rel and Sp1-like proteins in lipopolysaccharide-mediated activation of the HIV-1 LTR in macrophage
Journal name Journal of Inflammation   Check publisher's open access policy
ISSN 1476-9255
Publication date 1998-01-01
Sub-type Article (original research)
Open Access Status Not yet assessed
Volume 48
Issue 2
Start page 67
End page 83
Total pages 17
Place of publication London, United Kingdom
Publisher BioMed Central
Language eng
Subject 060405 Gene Expression (incl. Microarray and other genome-wide approaches)
Abstract The HIV-1 promoter was used as a model to identify transcription factors involved in LPS-dependent transcription in RAW 264 murine macrophages. Expression plasmids for Ets-2 and PU.1 trans-activated the HIV-1 LTR and recombinant PU.1 and an Ets-2 DNA binding domain/GST fusion protein bound to the 5' kappa B site of the HIV-1 enhancer. Ets-2 mRNA was LPS-inducible in RAW 264 cells and LPS stimulated phosphorylation of threonine 72 residue within the Ets-2 pointed domain. Induction of Ets-2 and other LPS-responsive transcription factors was also observed upon addition of plasmid DNA, which complicates interpretation of transient transfections. The proximal promoter region, containing two Sp1 sites, was also LPS-responsive. We propose that the kappa B elements and the tandem Sp1 sites act as LPS response elements and that kappa B-mediated LPS action involves Ets and rel factors.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Institute for Molecular Bioscience - Publications
 
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Created: Tue, 13 Jul 2010, 22:09:49 EST by Laura McTaggart on behalf of Institute for Molecular Bioscience