Plasmodium vivax synonymous substitution frequencies, evolution and population structure deduced from diversity in AMA 1 and MSP 1 genes

Figtree, Melanie, Pasay, Cielo J, Slade, Robert, Cheng, Qin, Cloonan, Nicole, Walker, John and Saul, Allan (2000) Plasmodium vivax synonymous substitution frequencies, evolution and population structure deduced from diversity in AMA 1 and MSP 1 genes. Molecular and Biochemical Parasitology, 108 1: 53-66. doi:10.1016/S0166-6851(00)00204-8


Author Figtree, Melanie
Pasay, Cielo J
Slade, Robert
Cheng, Qin
Cloonan, Nicole
Walker, John
Saul, Allan
Title Plasmodium vivax synonymous substitution frequencies, evolution and population structure deduced from diversity in AMA 1 and MSP 1 genes
Journal name Molecular and Biochemical Parasitology   Check publisher's open access policy
ISSN 0166-6851
Publication date 2000-04-01
Sub-type Article (original research)
DOI 10.1016/S0166-6851(00)00204-8
Volume 108
Issue 1
Start page 53
End page 66
Total pages 14
Place of publication Amsterdam, The Netherlands
Publisher Elsevier/North Holland
Language eng
Subject 0604 Genetics
Formatted abstract
Polymorphic regions of the genes encoding Plasmodium vivax apical membrane antigen 1 (PvAMA1) and P. vivax merozoite surface protein 1 (PvMSP1) were sequenced to examine population diversity both within and between geographical areas. Sequences were obtained for 219 isolates for PvAMA1 and for 175 isolates for PvMSP1 from Africa, China, India, Indonesia, Philippines, Papua New Guinea, Solomon Islands and Thailand. Over half of the isolates were obtained from different regions within the Philippines, and this was used to look at the diversity within a country. Sixty nine haplotypes and 22 polymorphic sites in a 414-bp region of PvAMA1 and 41 haplotypes and 34 polymorphic sites in a 249-bp fragment of PvMSP1 were detected. For both PvAMA1 and PvMSP1, four previously unreported polymorphic nucleotide positions were identified. Population analysis indicated that there were significant differences in allele frequencies between different regions but these differences were small compared to the diversity within populations (Fixation index, FST, of 0.126 and 0.078 for PvAMA1 and PvMSP1, respectively). PvAMA1 and PvMSP1 had similar nonsynonymous substitution frequencies but surprisingly, the synonymous substitution frequency for PvMSP1 was eight times the frequency for PvAMA1 suggesting that synonymous substitutions in at least PvAMA1 are not neutral.
Keyword malaria
Plasmodium vivax
apical membrane antigen 1
merozoite surface protein 1
population structure
synonymous
Merozoite Surface Protein-1
Apical Membrane Antigen-1
Circumsporozoite Protein
Sequence-Analysis
Dna-Sequences
Falciparum
Recombination
Polymorphism
Chloroquine
Infections
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Chemistry and Molecular Biosciences
 
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