AFLP linkage map of the Oomycete Phytophthora infestans

Vander Lee, Theo, de Witte, Ijfke, Drenth, André, Alfonso, Carlos and Govers, Francine (1997) AFLP linkage map of the Oomycete Phytophthora infestans. Fungal Genetics and Biology, 21 3: 278-291. doi:10.1006/fgbi.1997.0981

Author Vander Lee, Theo
de Witte, Ijfke
Drenth, André
Alfonso, Carlos
Govers, Francine
Title AFLP linkage map of the Oomycete Phytophthora infestans
Journal name Fungal Genetics and Biology   Check publisher's open access policy
ISSN 1087-1845
Publication date 1997
Sub-type Article (original research)
DOI 10.1006/fgbi.1997.0981
Volume 21
Issue 3
Start page 278
End page 291
Total pages 14
Publisher Academic Press
Language eng
Subject 060704 Plant Pathology
Formatted abstract
Here we present the first comprehensive genetic linkage map of the heterothallic oomycetous plant pathogenPhytophthora infestans.The map is based on polymorphic DNA markers generated by the DNA fingerprinting technique AFLP (Voset al.,1995,Nucleic Acids Res.23:4407–4414). AFLP fingerprints were made from single zoospore progeny and 73 F1 progeny from two field isolates ofP. infestans.The parental isolates appeared to be homokaryotic and diploid, their AFLP patterns were mitotically stable, and segregation ratios in the F1 progeny were largely Mendelian. In addition to 183 AFLP markers, 7 RFLP markers and the mating type locus were mapped. The linkage map comprises 10 major and 7 minor linkage groups covering a total of 827 cM. The major linkage groups are composed of markers derived from both parents, whereas the minor linkage groups contain markers from either the A1 or the A2 mating type parent. Non-Mendelian segregation ratios were found for the mating type locus and for 13 AFLP markers, all of which are located on the same linkage group as the mating type locus.
Keyword Phytophthora infestans
linkage map
plant pathogen
potato late blight
AFLP DNA fingerprinting
mating type
non-Mendelian segregation
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Biological Sciences Publications
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Citation counts: TR Web of Science Citation Count  Cited 115 times in Thomson Reuters Web of Science Article | Citations
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Created: Mon, 12 Jul 2010, 14:57:55 EST by Laura McTaggart on behalf of Faculty of Science