Quantitative SLM-based differential interference contrast imaging

McIntyre, Timothy J., Maurer, Christian, Fassl, Stephanie, Khan, Saranjam, Bernet, Stefan and Ritsch-Marte, Monika (2010) Quantitative SLM-based differential interference contrast imaging. Optics Express, 18 13: 14063-14078. doi:10.1364/OE.18.014063


Author McIntyre, Timothy J.
Maurer, Christian
Fassl, Stephanie
Khan, Saranjam
Bernet, Stefan
Ritsch-Marte, Monika
Title Quantitative SLM-based differential interference contrast imaging
Journal name Optics Express   Check publisher's open access policy
ISSN 1094-4087
Publication date 2010-06-21
Sub-type Article (original research)
DOI 10.1364/OE.18.014063
Open Access Status DOI
Volume 18
Issue 13
Start page 14063
End page 14078
Total pages 16
Place of publication Washington, DC, U.S.A.
Publisher Optical Society of America
Collection year 2011
Language eng
Abstract We describe the implementation of quantitative Differential Interference Contrast (DIC) Microscopy using a spatial light modulator (SLM) as a flexible Fourier filter in the optical path. The experimental arrangement allows for the all-electronic acquisition of multiple phase shifted DIC-images at video rates which are analyzed to yield the optical path length variation of the sample. The resolution of the technique is analyzed by retrieving the phase profiles of polystyrene spheres in immersion oil, and the method is then applied for quantitative imaging of biological samples. By reprogramming the diffractive structure displayed at the SLM it is possible to record the whole set of phase shifted DIC images simultaneously in different areas of the same camera chip. This allows for quantitative snap-shot imaging of a sample, which has applications for the investigation of dynamic processes. © 2010 Optical Society of America
Keyword Digital holographic microscopy
Spatial light-modulator
X-ray microscopy
Red-blood-cells
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Mathematics and Physics
Official 2011 Collection
 
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Citation counts: TR Web of Science Citation Count  Cited 16 times in Thomson Reuters Web of Science Article | Citations
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Created: Sun, 11 Jul 2010, 00:02:09 EST