Antibiotics for the preservation of koala (Phascolarctos cinereus) semen

Johnston, S. D., O'Boyle, D., Frost, A. J., McGowan, M. R., Tribe, A. and Higgins, D. (1998) Antibiotics for the preservation of koala (Phascolarctos cinereus) semen. Australian Veterinary Journal, 76 5: 335-338. doi:10.1111/j.1751-0813.1998.tb12362.x

Author Johnston, S. D.
O'Boyle, D.
Frost, A. J.
McGowan, M. R.
Tribe, A.
Higgins, D.
Title Antibiotics for the preservation of koala (Phascolarctos cinereus) semen
Journal name Australian Veterinary Journal   Check publisher's open access policy
ISSN 0005-0423
Publication date 1998-05
Sub-type Article (original research)
DOI 10.1111/j.1751-0813.1998.tb12362.x
Volume 76
Issue 5
Start page 335
End page 338
Total pages 4
Place of publication Carlton South, VIC
Publisher Australian Veterinary Association
Language eng
Subject 070206 Animal Reproduction
070707 Veterinary Microbiology (excl. Virology)
Formatted abstract
Aim To determine the normal microbial flora of the koala ejaculate and prepuce in order to select appropriate antibiotics for addition into diluents designed for the preservation of semen.

Procedure Bacteriological samples of the koala prepuce (n = 12) and ejaculate (n = 20) were submitted for microbial culture and sensitivity testing. Microbial flora of ejaculates collected by electroejaculation and artificial vagina were compared. The effects of varying concentrations of penicillin G and gentamicin on sperm motility and on the growth of bacteria in diluted semen stored at room temperature and 16°C over a 24 h period were investigated.

Results A range of bacteria was isolated from the koala prepuce and ejaculate. The predominant organisms in semen collected by electroejaculation and artificial vagina were Corynebacterium spp, none of which could be assigned to any recognised species. The addition of penicillin G and gentamicin to a PBS-based diluent at dose rates of 1000 to 2000 IU/mL and 100 to 200 m g/mL respectively, resulted in no adverse effect on sperm motility over a 24 h incubation period. Penicillin G (1000 IU/mL) and gentamicin (100 m g/mL) prevented growth of bacterial contaminants in diluted koala semen.

Conclusion By controlling the growth of bacteria in extended koala semen, penicillin G and gentamicin are likely to lengthen the period by which spermatozoa can be stored at 16°C and reduce the possibility of disease transmission during artificial insemination procedures.
Keyword bacteria
penicillin G
Corynebacterium spp.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Agriculture and Food Sciences
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Citation counts: TR Web of Science Citation Count  Cited 7 times in Thomson Reuters Web of Science Article | Citations
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Created: Wed, 07 Jul 2010, 16:20:47 EST by Laura McTaggart on behalf of Faculty Of Nat Resources, Agric & Veterinary Sc