Keratinocyte-driven contraction of reconstructed human skin

Chakrabarty, K. H., Heaton, M., Dalley, A. J., Dawson, R. A., Freedlander, E., Khaw, P. T. and Mac Neil, S. (2001) Keratinocyte-driven contraction of reconstructed human skin. Wound Repair and Regeneration, 9 2: 95-106. doi:10.1046/j.1524-475x.2001.00095.x


Author Chakrabarty, K. H.
Heaton, M.
Dalley, A. J.
Dawson, R. A.
Freedlander, E.
Khaw, P. T.
Mac Neil, S.
Title Keratinocyte-driven contraction of reconstructed human skin
Journal name Wound Repair and Regeneration   Check publisher's open access policy
ISSN 1067-1927
1524-475X
Publication date 2001
Year available 2001
Sub-type Article (original research)
DOI 10.1046/j.1524-475x.2001.00095.x
Volume 9
Issue 2
Start page 95
End page 106
Total pages 12
Editor Patricia A Hebda
Place of publication Wiley-Blackwell Publishing
Publisher New York, U.S.A.
Language eng
Subject 1103 Clinical Sciences
Abstract We have previously reported that reconstructed human skin, using deepidermized acellular sterilized dermis and allogeneic keratinocytes and fibroblasts, significantly contracts in vitro. Contracture of split skin grafts in burns injuries remains a serious problem and this in vitro model provides an opportunity to study keratinocyte/mesenchymal cell interactions and cell interactions with extracted normal human dermis. The aim of this study was to investigate the nature of this in vitro contraction and explore several approaches to prevent or reduce contraction. Three different methodologies for sterilization of the dermal matrix were examined: glycerol, ethylene oxide and a combination of glycerol and ethylene oxide. While the nature of the sterilization technique influenced the extent of contraction and thinner dermal matrices contracted proportionately more than thicker matrices, in all cases contraction was driven by the keratinocytes with relatively little influence from the fibroblasts. The contraction of the underlying dermis did not represent any change in tissue mass but rather a reorganization of the dermis which was rapidly reversed (within minutes) when the epidermal layer was removed. Pharmacological approaches to block contraction showed forskolin and mannose-6-phosphate to be ineffective and ascorbic acid-2-phosphate to exacerbate contraction. However, Galardin, a matrixmetalloproteinase inhibitor and keratinocyte conditioned media, both inhibited contraction. (WOUND REP REG 2001;9:95–106)
Keyword Human skin
Deepidermized acellular sterilized dermis
Allogeneic keratinocytes
In vitro contraction
Contraction prevention approach
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Centre for Clinical Research Publications
 
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Created: Fri, 02 Jul 2010, 10:06:23 EST by Ms Susana Macanawai on behalf of Faculty Of Health Sciences