A porcine model of hypertrophic deep dermal partial thickness burn for wound healing studies

Cuttle, Leila, Kempf, Margit, Phillips, Gael E., Mill, Julie, Hayes, Mark T., Fraser, John F., Wang, Xue-Qing and Kimble, Roy M. (2006) A porcine model of hypertrophic deep dermal partial thickness burn for wound healing studies. Burns, 32 7: 806-820. doi:10.1016/j.burns.2006.02.023

Author Cuttle, Leila
Kempf, Margit
Phillips, Gael E.
Mill, Julie
Hayes, Mark T.
Fraser, John F.
Wang, Xue-Qing
Kimble, Roy M.
Title A porcine model of hypertrophic deep dermal partial thickness burn for wound healing studies
Journal name Burns   Check publisher's open access policy
ISSN 0305-4179
Publication date 2006
Year available 2006
Sub-type Article (original research)
DOI 10.1016/j.burns.2006.02.023
Volume 32
Issue 7
Start page 806
End page 820
Total pages 15
Place of publication Oxford , United Kingdom
Publisher Elseiver Science
Language eng
Subject 11 Medical and Health Sciences
Abstract We developed a reproducible model of deep dermal partial thickness burn injury in juvenile Large White pigs. The contact burn is created using water at 92 °C for 15 s in a bottle with the bottom replaced with plastic wrap. The depth of injury was determined by a histopathologist who examined tissue sections 2 and 6 days after injury in a blinded manner. Upon creation, the circular wound area developed white eschar and a hyperaemic zone around the wound border. Animals were kept for 6 weeks or 99 days to examine the wound healing process. The wounds took between 3 and 5 weeks for complete re-epithelialisation. Most wounds developed contracted, purple, hypertrophic scars. On measurement, the thickness of the burned skin was approximately 1.8 times that of the control skin at week 6 and approximately 2.2 times thicker than control skin at 99 days after injury. We have developed various methods to assess healing wounds, including digital photographic analysis, depth of organising granulation tissue, immunohistochemistry, electron microscopy and tensiometry. Immunohistochemistry and electron microscopy showed that our porcine hypertrophic scar appears similar to human hypertrophic scarring. The development of this model allows us to test and compare different treatments on burn wounds.
Keyword Burn injuries
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ
Additional Notes Paper presented at the 4th Annual International Paediatric Burns Video Conference Brisbane, Australia (7 centres in Canada, USA, UK, Australia) November, 2004

Document type: Journal Article
Sub-type: Article (original research)
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