Enhanced effects of low molecular weight heparin intercalated with layered double hydroxide nanoparticles on rat vascular smooth muscle cells

Gu, Zi, Rolfe, Barbara E., Xu, Zhi P., Thomas, Anita C., Campbell, Julie H. and Lu, Gao Q.M. (2010) Enhanced effects of low molecular weight heparin intercalated with layered double hydroxide nanoparticles on rat vascular smooth muscle cells. Biomaterials, 31 20: 5455-5462. doi:10.1016/j.biomaterials.2010.03.050

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Author Gu, Zi
Rolfe, Barbara E.
Xu, Zhi P.
Thomas, Anita C.
Campbell, Julie H.
Lu, Gao Q.M.
Title Enhanced effects of low molecular weight heparin intercalated with layered double hydroxide nanoparticles on rat vascular smooth muscle cells
Journal name Biomaterials   Check publisher's open access policy
ISSN 0142-9612
1878-5905
Publication date 2010-07
Sub-type Article (original research)
DOI 10.1016/j.biomaterials.2010.03.050
Volume 31
Issue 20
Start page 5455
End page 5462
Total pages 8
Place of publication Oxford, England
Publisher Elsevier
Collection year 2011
Language eng
Abstract Surgical procedures to remove atherosclerotic lesions and restore blood flow also injure the artery wall, promoting vascular smooth muscle cell (SMC) phenotypic change, migration, proliferation, matrix production and ultimately, restenosis of the artery. Hence identification of effective anti-restenotic strategies is a high priority in cardiovascular research, and SMCs are a key target for intervention. This paper presents the in vitro study of layered double hydroxides (LDHs) as drug delivery system for an anti-restenotic drug (low molecular weight heparin, LMWH). The cytotoxicity tests showed that LDH itself had very limited toxicity at concentrations below 50μg/mL over 6-day incubation. LDH nanoparticles loaded with LMWH (LMWH-LDHs) were prepared and tested on rat vascular SMCs. When conjugated to LDH particles, LMWH enhanced its ability to inhibit SMC proliferation and migration, with greater than above 60% reduction compared with the control (growth medium) over 3 or 7-day incubation. Cellular uptake studies showed that compared with LMWH alone, LMWH-LDH hybrids were internalized by SMCs more rapidly, and uptake was sustained over a longer time, possibly revealing the mechanisms underlying the enhanced biological function of LMWH-LDH. The results suggest the potential of LMWH-LDH as an efficient anti-restenotic drug for clinical application. © 2010 Elsevier Ltd.
Keyword Layered double hydroxides
Low molecular weight heparin
Rat vascular smooth muscle cells
Proliferation
Migration
Cellular uptake
Drug-delivery
Coronary Angioplasty
Cellular Proliferation
Arterial Injury
Quantum Dots
Restenosis
Growth
Prevention
Migration
Nanohybrids
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Chemical Engineering Publications
Official 2011 Collection
 
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Citation counts: TR Web of Science Citation Count  Cited 35 times in Thomson Reuters Web of Science Article | Citations
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Created: Sun, 27 Jun 2010, 00:09:48 EST