Purification and conformational analysis of a key exopolysaccharide component of mixed culture aerobic sludge granules

Seviour, Thomas, Donose, Bogdan C., Pijuan, Maite and Yuan, Zhiguo (2010) Purification and conformational analysis of a key exopolysaccharide component of mixed culture aerobic sludge granules. Environmental Science & Technology, 44 12: 4729-4734. doi:10.1021/es100362b


Author Seviour, Thomas
Donose, Bogdan C.
Pijuan, Maite
Yuan, Zhiguo
Title Purification and conformational analysis of a key exopolysaccharide component of mixed culture aerobic sludge granules
Journal name Environmental Science & Technology   Check publisher's open access policy
ISSN 0013-936X
Publication date 2010-06-15
Sub-type Article (original research)
DOI 10.1021/es100362b
Volume 44
Issue 12
Start page 4729
End page 4734
Total pages 6
Editor Jerald Schnoor
Place of publication Washington, DC, U.S.A.
Publisher American Chemical Society
Collection year 2011
Language eng
Abstract The application of aerobic sludge granules in wastewater treatment could increase the intensity of wastewater treatment processes because of their greater density and size relative to conventional sludge flocs. It has been suggested that granules are distinguished from flocs by gel forming exopolysaccharides. In this study, evidence is presented linking a specific exopolysaccharide component with granule extracellular polymeric substance (EPS) gelation. Granular EPS comprised three components: high-molecular-weight (MW) exopolysaccharide, medium-MW proteins and glycosides, and low-MW proteins and glycosides. The high-MW fraction was separated by fractional precipitation and preparatory-scale gel permeation chromatography (GPC). The MW profile of this fraction appears to be exclusively attributable to high-MW polysaccharide. The exopolysaccharide exists as a gel at normal wastewater treatment operating pH (i.e., 6.0−8.5), whereas the low/medium-MW material does not. Conformational analysis by atomic force microscopy (AFM) of the dried material showed that the polysaccharide forms pearl-necklace-like, intramolecularly condensed structures when dissolved in Milli-Q water and partially relaxed helical aggregates when in alkali solution. Consequently, the gel-forming property of EPS in the aerobic sludge granules tested is probably associated with high-MW polysaccharide components.
Keyword Extracellular polymeric substances
Atomic-force microscopy
Size-exclusion chromatography
Activated-sludge
Waste-water
Stability
Polysaccharides
Chain
Hyaluronan
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Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Official 2011 Collection
 
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Created: Sun, 27 Jun 2010, 10:07:58 EST