Local immune response against larvae of Rhipicephalus (Boophilus) microplus in Bos taurus indicus and Bos taurus taurus cattle

Constantinoiu, C. C., Jackson, L. A., Jorgensen, W. K., Lew-Tabor, A. E., Piper, E. K., Mayer, D. G., Venus, B. and Jonsson, N. N. (2010) Local immune response against larvae of Rhipicephalus (Boophilus) microplus in Bos taurus indicus and Bos taurus taurus cattle. International Journal for Parasitology, 40 7: 865-875. doi:10.1016/j.ijpara.2010.01.004


Author Constantinoiu, C. C.
Jackson, L. A.
Jorgensen, W. K.
Lew-Tabor, A. E.
Piper, E. K.
Mayer, D. G.
Venus, B.
Jonsson, N. N.
Title Local immune response against larvae of Rhipicephalus (Boophilus) microplus in Bos taurus indicus and Bos taurus taurus cattle
Formatted title
Local immune response against larvae of Rhipicephalus (Boophilus) microplus in Bos taurus indicus and Bos taurus taurus cattle
Journal name International Journal for Parasitology   Check publisher's open access policy
ISSN 0020-7519
Publication date 2010-06
Sub-type Article (original research)
DOI 10.1016/j.ijpara.2010.01.004
Open Access Status
Volume 40
Issue 7
Start page 865
End page 875
Total pages 11
Place of publication United Kingdom
Publisher Elsevier
Collection year 2011
Language eng
Subject 0605 Microbiology
0608 Zoology
0707 Veterinary Sciences
Formatted abstract
Bos taurus indicus cattle are less susceptible to infestation with Rhipicephalus (Boophilus) microplus than Bos taurus taurus cattle but the immunological basis of this difference is not understood. We compared the dynamics of leukocyte infiltrations (T cell subsets, B cells, major histocompatibility complex (MHC) class II-expressing cells, granulocytes) in the skin near the mouthparts of larvae of R. microplus in B. t. indicus and B. t. taurus cattle. Previously naïve cattle were infested with 50,000 larvae (B. t. indicus) or 10,000 larvae (B. t. taurus) weekly for 6 weeks. One week after the last infestation all of the animals were infested with 20,000 larvae of R. microplus. Skin punch biopsies were taken from all animals on the day before the primary infestation and from sites of larval attachment on the day after the first, second, fourth and final infestations. Infiltrations with CD3+, CD4+, CD8+ and γδ T cells followed the same pattern in both breeds, showing relatively little change during the first four weekly infestations, followed by substantial increases at 7 weeks post-primary infestation. There was a tendency for more of all cell types except granulocytes to be observed in the skin of B. t. indicus cattle but the differences between the two breeds were consistently significant only for γδ T cells. Granulocyte infiltrations increased more rapidly from the day after infestation and were higher in B. t. taurus cattle than in B. t. indicus. Granulocytes and MHC class II-expressing cells infiltrated the areas closest to the mouthparts of larvae. A large volume of granulocyte antigens was seen in the gut of attached, feeding larvae.
Keyword Rhipicephalus (Boophilus) microplus
Host immune response
Bos taurus taurus
Bos taurus indicus
Immunohistochemistry
Skin
Cattle
Delta T-Cells
Mhc Class-Ii
Host-Resistance
Gamma-Delta
Monoclonal-Antibodies
Feeding Processes
Gene-Expression
Tick
Canestrini
Skin
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
Queensland Alliance for Agriculture and Food Innovation
School of Veterinary Science Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 19 times in Thomson Reuters Web of Science Article | Citations
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Created: Sun, 27 Jun 2010, 00:06:46 EST