Micro-CT analysis of matrix-type drug delivery devices and correlation with protein release behaviour

Wang, Yiwei, Wertheim, David F., Jones, Allan S., Chang, Hsin-I and Coombes, Allan G.A. (2010) Micro-CT analysis of matrix-type drug delivery devices and correlation with protein release behaviour. Journal of Pharmaceutical Sciences, 99 6: 2854-2862. doi:10.1002/jps.22027


Author Wang, Yiwei
Wertheim, David F.
Jones, Allan S.
Chang, Hsin-I
Coombes, Allan G.A.
Title Micro-CT analysis of matrix-type drug delivery devices and correlation with protein release behaviour
Journal name Journal of Pharmaceutical Sciences   Check publisher's open access policy
ISSN 1520-6017
0022-3549
Publication date 2010-06
Year available 2009
Sub-type Article (original research)
DOI 10.1002/jps.22027
Volume 99
Issue 6
Start page 2854
End page 2862
Total pages 9
Place of publication Hoboken, NJ, U.S.A
Publisher Wiley-Liss
Collection year 2011
Language eng
Abstract A series of matrix-type drug delivery devices comprising a continuous phase of microporous poly(ε-caprolactone) (PCL) and a dispersed phase of protein particles (gelatin) with defined size ranges (45-90, 90-125 and 125-250 mm) were produced by rapidly cooling suspensions in dry ice followed by solvent extraction from the hardened material. High protein loadings (38-44%, w/w) were achieved and highly efficient protein release (90% of the initial load) was obtained over time periods of 3-11 days depending on particle loading and size range. The duration of protein release was extended from 3 to 11 days reducing the protein load. Quantitative analysis of Micro-CT images identified a three to four times increase in the population of sub-40 μm pores in those matrices which gave rise to accelerated protein release in 24 h (40% rising to 80%) and reduced duration of protein release (11-3 days). Formation of a high density of channels and fissures (connects) between the particles is indicated, which facilitate fluid ingress and diffusion of solubilised protein molecules. Micro-CT analysis also confirmed the uniformity of particle distribution in the matrices and provided measurements of macroporosity within 5-30% of the theoretical value for materials displaying irregular shaped macropores larger than 90 μm. These findings demonstrate the utility of Micro-CT for optimising the formulation and performance of matrix-type delivery devices for macromolecular entities. © 2009 Wiley-Liss, Inc. and the American Pharmacists Association.
Keyword polycaprolactone
Microporous
Matrix
Micro-CT
Protein delivery
Polymeric scaffolds
Permeability
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Article first published online: 14 December, 2009.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
School of Pharmacy Publications
 
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Created: Sun, 20 Jun 2010, 00:03:57 EST