DNA is taken up by root hairs and pollen, and stimulates root and pollen tube growth

Paungfoo-Lonhienne, Chanyarat, Lonhienne, Thierry G. A., Mudge, Stephen R., Schenk, Peer M., Christie, Michael, Carroll, Bernard J. and Schmidt, Susanne (2010) DNA is taken up by root hairs and pollen, and stimulates root and pollen tube growth. Plant Physiology, 153 2: 799-805. doi:10.1104/pp.110.154963

Author Paungfoo-Lonhienne, Chanyarat
Lonhienne, Thierry G. A.
Mudge, Stephen R.
Schenk, Peer M.
Christie, Michael
Carroll, Bernard J.
Schmidt, Susanne
Title DNA is taken up by root hairs and pollen, and stimulates root and pollen tube growth
Journal name Plant Physiology   Check publisher's open access policy
ISSN 0032-0889
Publication date 2010-06
Sub-type Article (original research)
DOI 10.1104/pp.110.154963
Open Access Status
Volume 153
Issue 2
Start page 799
End page 805
Total pages 7
Place of publication Rockville, MD, United States
Publisher American Society of Plant Biologists
Collection year 2011
Language eng
Subject C1
829899 Environmentally Sustainable Plant Production not elsewhere classified
060702 Plant Cell and Molecular Biology
Formatted abstract
Phosphorus (P) enters roots as inorganic phosphate (Pi) derived from organic and inorganic P compounds in the soil. Nucleic acids can support plant growth as the sole source of P in axenic culture but are thought to be converted into Pi by plant-derived nucleases and phosphatases prior to uptake. Here, we show that a nuclease-resistant analog of DNA is taken up by plant cells. Fluorescently labeled S-DNA of 25 bp, which is protected against enzymatic breakdown by its phosphorothioate backbone, was taken up and detected in root cells including root hairs and pollen tubes. These results indicate that current views of plant P acquisition may have to be revised to include uptake of DNA into cells. We further show that addition of DNA to Pi-containing growth medium enhanced the growth of lateral roots and root hairs even though plants were P replete and had similar biomass as plants supplied with Pi only. Exogenously supplied DNA increased length growth of pollen tubes, which were studied because they have similar elongated and polarized growth as root hairs. Our results indicate that DNA is not only taken up and used as a P source by plants, but ironically and independent of Pi supply, DNA also induces morphological changes in roots similar to those observed with P limitation. This study provides, to our knowledge, first evidence that exogenous DNA could act nonspecifically as signaling molecules for root development. © 2010 American Society of Plant Biologists.
Keyword Phosphate transporter gene
Organic Phosphorus
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

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Created: Sun, 20 Jun 2010, 00:01:51 EST