Microsatellite detection of donor-derived sperm DNA following germ cell transplantation in cattle

Stockwell, Sally, Herrid, Muren, Davey, Rhonda, Brownlee, Alan, Hutton, Keryn and Hill, Jonathan R. (2009) Microsatellite detection of donor-derived sperm DNA following germ cell transplantation in cattle. Reproduction, Fertility and Development, 21 3: 462-468. doi:10.1071/RD08130


Author Stockwell, Sally
Herrid, Muren
Davey, Rhonda
Brownlee, Alan
Hutton, Keryn
Hill, Jonathan R.
Title Microsatellite detection of donor-derived sperm DNA following germ cell transplantation in cattle
Journal name Reproduction, Fertility and Development   Check publisher's open access policy
ISSN 1031-3613
Publication date 2009-11
Sub-type Article (original research)
DOI 10.1071/RD08130
Volume 21
Issue 3
Start page 462
End page 468
Total pages 7
Editor Tony Flint
Place of publication East Melbourne, Vic.
Publisher CSIRO in co-operation with the Australian Academy of Science
Collection year 2010
Language eng
Subject C1
0707 Veterinary Sciences
Abstract Although autologous and heterologous transplantation has resulted in colonisation of recipient testes in cattle, the ability of the transplanted spermatogonial stem cells to complete spermatogenesis has not yet been determined. The objective of the present study was to identify and validate microsatellite markers that can distinguish the genotype of different individuals and therefore can be used to detect the presence of donor DNA in recipient semen samples. In a previous study by this group, successful colonisation of recipient testes by heterologous transfer using a fluorescent dye was shown. In the present work, some of the same recipient animals were investigated further to monitor donor-derived sperm production. The bovine microsatellite detection method was developed specifically to test the ejaculates of the recipients and can also be used to pre-match individuals before germ cell transplantation. Semen was collected from the recipients 52–98 weeks after transfer and the presence of donor DNA in the samples was determined using microsatellite markers. In one of the recipients, all collected semen samples were shown to be positive for donor-derived cells; however, the percentage of donor spermatozoa in the recipient ejaculate declined with time. The donor DNA was also detected in both single cell suspensions and testis tissue from this recipient. These results demonstrate for the first time that testicular germ cell transplantation between different breeds of cattle is feasible and the recipients thereof are able to produce spermatozoa of donor origin. This technology has potential applications in livestock breeding systems and may provide an alternative to artificial insemination.
Keyword donor sperm
spermatogenesis
Q-Index Code C1
Q-Index Status Confirmed Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
School of Veterinary Science Publications
 
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Created: Fri, 18 Jun 2010, 10:24:06 EST by Matthew Lamb on behalf of Faculty Of Nat Resources, Agric & Veterinary Sc