Optimized extended depth of focus simulated analysis for confocal microscopy

Duadi, Hamootal, Margalit, Ofer, Zalevsky, Zeev and Sarafis, Vassilios (2010) Optimized extended depth of focus simulated analysis for confocal microscopy. Journal of the Optical Society of America A: Optics and Image Science, and Vision, 27 6: 1378-1384. doi:10.1364/JOSAA.27.001378


Author Duadi, Hamootal
Margalit, Ofer
Zalevsky, Zeev
Sarafis, Vassilios
Title Optimized extended depth of focus simulated analysis for confocal microscopy
Journal name Journal of the Optical Society of America A: Optics and Image Science, and Vision   Check publisher's open access policy
ISSN 1084-7529
1520-8532
Publication date 2010-06-01
Sub-type Article (original research)
DOI 10.1364/JOSAA.27.001378
Volume 27
Issue 6
Start page 1378
End page 1384
Total pages 7
Place of publication Washington, DC, United States
Publisher Optical Society of America
Collection year 2011
Language eng
Abstract In imaging systems and especially in confocal microscopy systems there is a trade-off between the lateral resolution and the obtained depth of focus. The use of complex pupils to improve the lateral resolution by engineering the point spread function is a common approach; however the lateral improvement reduces the effective depth of focus and therefore the fluorescence efficiency. In this work we analytically develop an optimized approach for obtaining a complex pupil with an extended depth of focus. The proposed solution is numerically applied and tested in designing an improved focal depth in confocal microscope configuration. © 2010 Optical Society of America.
Keyword Fluorescence microscopy
Focal depth
Field
Illumination
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Mathematics and Physics
Official 2011 Collection
 
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Created: Sun, 13 Jun 2010, 10:02:29 EST