Characterization of multidrug-resistant Escherichia coli isolated from extraintestinal clinical infections in animals

Gibson, Justine S., Cobbold, Rowland N. and Trott, Darren J. (2010) Characterization of multidrug-resistant Escherichia coli isolated from extraintestinal clinical infections in animals. Journal of Medical Microbiology, 59 5: 592-598. doi:10.1099/jmm.0.018002-0


Author Gibson, Justine S.
Cobbold, Rowland N.
Trott, Darren J.
Title Characterization of multidrug-resistant Escherichia coli isolated from extraintestinal clinical infections in animals
Formatted title
Characterization of multidrug-resistant Escherichia coli isolated from extraintestinal clinical infections in animals
Journal name Journal of Medical Microbiology   Check publisher's open access policy
ISSN 0022-2615
1473-5644
Publication date 2010-05-01
Sub-type Article (original research)
DOI 10.1099/jmm.0.018002-0
Volume 59
Issue 5
Start page 592
End page 598
Total pages 7
Editor Charles Penn
Place of publication Reading, U.K.
Publisher Society for General Microbiology
Collection year 2011
Language eng
Formatted abstract
Multidrug-resistant (MDR) Escherichia coli causes extraintestinal infections in both humans and animals. This study aimed to determine whether MDR E. coli isolates cultured from extraintestinal infections in several animal species were clonal and crossed host-species boundaries, as suggested by initial characterization of a subset of canine and human isolates, or whether they represented a diverse group of host-specific strains. Isolates were obtained either from The University of Queensland Veterinary Diagnostic Laboratory or from an independent diagnostic laboratory between October 1999 and December 2007. Ninety-six MDR E. coli isolates cultured from extraintestinal clinical infections in 55 animals comprising dogs (n=45), cats (n=5), horses (n=4) and a koala (n=1) were analysed by phylogenetic grouping, antimicrobial susceptibility testing and PFGE. The isolates were cultured from the urinary tract (n=61), reproductive tract (n=11), wounds (n=11), surgical site infections (n=4) and other sites (n=9). Isolates from the same E. coli phylogenetic group with 100 % PFGE similarity and the same antimicrobial susceptibility pattern were considered to be repeat clones and excluded from further analysis. Three of the four E. coli phylogenetic groups (A, n=19; B1, n=8; and D, n=49) were represented. Analysis of PFGE similarity identified clusters of related phylogenetic group A isolates [clonal group (CG) 1] and group D isolates (CG2 and CG3), with the remainder of the isolates demonstrating diversity. The majority of CG2 isolates contained a plasmid-borne AmpC β-lactamase, imparting resistance to cefoxitin and third-generation cephalosporins, and were obtained between 2000 and 2003. CG3 isolates were sensitive to these antimicrobial agents and appeared to replace CG2 isolates as the dominant clones from 2003 to 2007. Apart from several canine and feline isolates that demonstrated clonality, PFGE profiles tended to be divergent across species. Whilst MDR E. coli isolates from extraintestinal infections in different animal species are diverse, some dominant CGs may persist over several years.
© 2010 SGM
Keyword Urinary-tract-infections
Colonization patterns
Virulence genotypes
Household members
Dogs
Identification
Emergence
Strains
Humans
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
School of Veterinary Science Publications
 
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Created: Sun, 06 Jun 2010, 10:04:11 EST