The effect of strain rate on the precision of penetration of short densely-packed microprojection array patches coated with vaccine

Crichton, Michael L., Ansaldo, Alexander, Chen, Xianfeng, Prow, Tarl W., Fernando, Germain J. P. and Kendall, Mark A. F. (2010) The effect of strain rate on the precision of penetration of short densely-packed microprojection array patches coated with vaccine. Biomaterials, 31 16: 4562-4572. doi:10.1016/j.biomaterials.2010.02.022


Author Crichton, Michael L.
Ansaldo, Alexander
Chen, Xianfeng
Prow, Tarl W.
Fernando, Germain J. P.
Kendall, Mark A. F.
Title The effect of strain rate on the precision of penetration of short densely-packed microprojection array patches coated with vaccine
Journal name Biomaterials   Check publisher's open access policy
ISSN 0142-9612
1878-5905
Publication date 2010-06
Sub-type Article (original research)
DOI 10.1016/j.biomaterials.2010.02.022
Volume 31
Issue 16
Start page 4562
End page 4572
Total pages 11
Place of publication Oxford, United Kingdom
Publisher Elsevier
Collection year 2011
Language eng
Subject 1107 Immunology
Formatted abstract
If skin's non-linear viscoelastic properties are mechanically exploited for precise antigen placement, there is tremendous promise for improved vaccines. To achieve this, we designed a Nanopatch™—densely packed micro-nanoprojections (>20,000/cm2) to directly deposit antigen to large numbers of epidermal Langerhans cells and dermal dendritic cells. Here, we controllably applied our Nanopatches™ with discrete conditions between peak strain rates of ~100 s−1–7000 s−1 and quantified resulting penetration depths, delivery payloads and skin mechanics. Increasing the strain rate of application, we overcame key skin variability, achieving increases in both projection penetration depth (by over 50% length) and area coverage of a full array (from 50% to 100%). This delivery depth precision opens the way for more fully utilizing the skin's immune function. Furthermore, we yielded new insights on mechanical behaviour of skin, including: 1) internal skin property changes that could affect/facilitate penetration; 2) projection design to dictate penetration depth; 3) puncture mechanics of skin in this strain rate range. Indeed, we show delivery of a model vaccine using our tested range of strain rates achieved functionally relevant tunable systemic antibody generation in mice. These findings could be of great utility in extending skin strata vaccine targeting approaches to human use.
© 2010 Elsevier Ltd. All rights reserved.
Keyword Skin
Drug delivery
Mechanical properties
Vaccine
Microneedles
Human skin
Mechanical-properties
Ballistic delivery
Microneedle insertion
Langerhans cells
Stratum-corneum
Dendritic cells
Human dermis
Mouse
Microparticles
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

 
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Created: Sun, 30 May 2010, 00:07:32 EST