Simultaneous measurement of aldosterone and cortisol by high-performance liquid chromatography-tandem mass spectrometry: Application to dehydration-rehydration studies

Taylor, Paul J., van Rosendal, Simon P., Coombes, Jeff S., Gordon, Richard D. and Stowasser, Michael (2010) Simultaneous measurement of aldosterone and cortisol by high-performance liquid chromatography-tandem mass spectrometry: Application to dehydration-rehydration studies. Journal of Chromatography B-analytical Technologies In The Biomedical And Life Sciences, 878 15-16: 1195-1198. doi:10.1016/j.jchromb.2010.03.033

Attached Files (Some files may be inaccessible until you login with your UQ eSpace credentials)
Name Description MIMEType Size Downloads
UQ205635.pdf HERDC Checklist - not publicly available application/pdf 48.07KB 0

Author Taylor, Paul J.
van Rosendal, Simon P.
Coombes, Jeff S.
Gordon, Richard D.
Stowasser, Michael
Title Simultaneous measurement of aldosterone and cortisol by high-performance liquid chromatography-tandem mass spectrometry: Application to dehydration-rehydration studies
Journal name Journal of Chromatography B-analytical Technologies In The Biomedical And Life Sciences   Check publisher's open access policy
ISSN 1570-0232
1873-376X
Publication date 2010-05-01
Sub-type Article (original research)
DOI 10.1016/j.jchromb.2010.03.033
Volume 878
Issue 15-16
Start page 1195
End page 1198
Total pages 4
Editor G. Hopfgartner
H. T. Karnes
R. Bischoff
Place of publication Amsterdam, The Netherlands; New York, U.S.A.
Publisher Elsevier
Collection year 2011
Language eng
Formatted abstract
Aldosterone and cortisol are useful biomarkers of dehydration and stress, respectively. The aim of this study was to develop an HPLC-tandem mass spectrometric method for the simultaneous measurement of aldosterone and cortisol in human plasma that could be applied to the study of athletes undergoing exercise and rehydration. Samples were prepared and analysed using an on-line sample preparation/HPLC system coupled to a triple quadrupole tandem-mass spectrometer. Samples (200 μL) were pre-treated and extracted on Hysphere C18 HD cartridges (7 μm, Spark Holland). Chromatography was performed on a Sunfire C18 analytical column (50 mm × 3.0 mm, 3 μm, Waters) under isocratic conditions at a flow rate of 0.3 mL/min. The mobile phase consisted of 35% acetonitrile/water. Mass spectrometric detection was by selected reaction monitoring using negative electrospray ionization conditions. The assay had an analytical range of 25-500 pg/mL and 25-500 ng/mL for aldosterone and cortisol, respectively (r2 > 0.992, n = 22). Inter-day accuracy and imprecision for quality control samples was 99.4-106% and <16%, respectively (n = 10). In a study of nine human subjects, both aldosterone and cortisol concentrations reflected the expected physiological responses to dehydration, rehydration and exercise when measured by this method. The reported method is suitable to facilitate the study of athletes undergoing dehydration and rehydration protocols.
© 2010 Elsevier B.V. All rights reserved.
Keyword Aldosterone
Cortisol
Mass spectrometry
Exercise
Dehydration
Stress
Exercise heat stress
Chemiluminescence-Immunossay
Endocrine response
Human serum
Plasma
Extraction
Radioimmunoassay
Hypohydration
Exposure
Hormones
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
School of Medicine Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 14 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 17 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Sun, 30 May 2010, 00:03:37 EST