A method for rapid, ligation-independent reformatting of recombinant monoclonal antibodies

Jones, Martina L., Seldon, Therese, Smede, Matthew, Linville, Ashleigh, Chin, David Y., Barnard, Ross, Mahler, Stephen M., Munster, David, Hart, Derek, Gray, Peter P. and Munro, Trent P. (2010) A method for rapid, ligation-independent reformatting of recombinant monoclonal antibodies. Journal of Immunological Methods, 354 1-2: 85-90. doi:10.1016/j.jim.2010.02.001

Author Jones, Martina L.
Seldon, Therese
Smede, Matthew
Linville, Ashleigh
Chin, David Y.
Barnard, Ross
Mahler, Stephen M.
Munster, David
Hart, Derek
Gray, Peter P.
Munro, Trent P.
Title A method for rapid, ligation-independent reformatting of recombinant monoclonal antibodies
Journal name Journal of Immunological Methods   Check publisher's open access policy
ISSN 0022-1759
Publication date 2010-03-31
Sub-type Article (original research)
DOI 10.1016/j.jim.2010.02.001
Volume 354
Issue 1-2
Start page 85
End page 90
Total pages 6
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Collection year 2011
Language eng
Formatted abstract
Recombinant monoclonal antibodies currently dominate the protein biologics marketplace. The path from target antigen discovery and screening, to a recombinant therapeutic antibody can be time-consuming and laborious. We describe a set of expression vectors, termed mAbXpress, that enable rapid and sequence-independent insertion of antibody variable regions into human constant region backbones. This method takes advantage of the In Fusion™ cloning system from Clontech, which allows ligation-free, high-efficiency insertion of the variable region cassette without the addition of extraneous amino acids. These modular vectors simplify the antibody reformatting process during the preliminary evaluation of therapeutic or diagnostic candidates. The resulting constructs can be used directly for transient or amplifiable, stable expression in mammalian cells. The effectiveness of this method was demonstrated by the creation of a functional, fully human anti-human CD83 monoclonal antibody.
Crown Copyright © 2010 Published by Elsevier B.V. All rights reserved.
Keyword Recombinant monoclonal antibodies
Phage display
CHO cells
Antibody reformatting
Antibody discovery
Phage display libraries
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

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Created: Sun, 30 May 2010, 00:02:57 EST