In vivo gene expression in granulosa cells during pig terminal follicular development

Bonnet, A., Lê Cao, K. A., SanCristobal, M., Benne, F., Robert-Granié, C., Law-So, G., Fabre, S., Besse, P., De Billy, E., Quesne, H., Hatey, F. and Tosser-Klopp, G. (2008) In vivo gene expression in granulosa cells during pig terminal follicular development. Reproduction, 136 2: 211-224. doi:10.1530/REP-07-0312


Author Bonnet, A.
Lê Cao, K. A.
SanCristobal, M.
Benne, F.
Robert-Granié, C.
Law-So, G.
Fabre, S.
Besse, P.
De Billy, E.
Quesne, H.
Hatey, F.
Tosser-Klopp, G.
Title In vivo gene expression in granulosa cells during pig terminal follicular development
Journal name Reproduction   Check publisher's open access policy
ISSN 1470-1626
1741-7899
Publication date 2008-08
Sub-type Article (original research)
DOI 10.1530/REP-07-0312
Volume 136
Issue 2
Start page 211
End page 224
Total pages 14
Place of publication Bristol, U.K.
Publisher BioScientifica
Language eng
Subject 0601 Biochemistry and Cell Biology
1116 Medical Physiology
Abstract Ovarian antral follicular development is clearly dependent on pituitary gonadotrophins FSH and LH. Although the endocrine mechanism that controls ovarian folliculogenesis leading to ovulation is quite well understood, the detailed mechanisms and molecular determinants in the different follicular compartments remain to be clarified. The aim of this study was to identify the genes differentially expressed in pig granulosa cells along the terminal ovarian follicle growth, to gain a comprehensive view of these molecular mechanisms. First, we developed a specific micro-array using cDNAs from suppression subtractive hybridization libraries (345 contigs) obtained by comparison of three follicle size classes: small, medium and large antral healthy follicles. In a second step, a transcriptomic analysis using cDNA probes from these three follicle classes identified 79 differentially expressed transcripts along the terminal follicular growth and 26 predictive genes of size classes. The differential expression of 18 genes has been controlled using real-time PCR experiments validating the micro-array analysis. Finally, the integration of the data using Ingenuity Pathways Analysis identified five gene networks providing descriptive elements of the terminal follicular development. Specifically, we observed: (1) the down-expression of ribosomal protein genes, (2) the genes involved in lipid metabolism and (3) the down-expression of cell morphology and ion-binding genes. In conclusion, this study gives new insight into the gene expression during pig terminal follicular growth in vivo and suggested, in particular, a morphological change in pig granulosa cells accompanying terminal follicular growth. © 2008 Society for Reproduction and Fertility.
Keyword Gene expression regulation
Gene identification
Gene regulatory network
Gene expression profiling
Q-Index Code C1
Q-Index Status Provisional Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Institute for Molecular Bioscience - Publications
 
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Created: Fri, 28 May 2010, 12:35:24 EST by Mary-Anne Marrington on behalf of Institute for Molecular Bioscience